Senecavirus A (SVA) PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1 – 2 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Porcine
Specimen
Name
Container
Minimum Amount
Vesicle fluid
Sealed, Leakproof Container
Vesicle swab
Viral transport medium or saline
Vesicle tissue
Sealed, Leakproof Container
Oral Fluid
Sealed, Leakproof container
2-3 ml
Serum
Sealed, Leakproof Conainter
1-2 ml
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
ORAL FLUID - Submit oral fluid in a leakproof container (i.e. plastic conical tube) with appropriate labeling.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
ORAL FLUID - Ensure the container is securely sealed and labeled.
Additional Information
If lesions suggest a foreign animal disease (e.g., FMD), you must contact your state animal health official before collecting or submitting specimens.- Indiana Animal Disease Diagnostic Laboratory Home
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