Aerobic Culture - Listeria

Section: Bacteriology

Fee: $45.00

Turnaround Time: 5 – 7 business days

Days Test Started: Monday

Species: Bovine, Caprine, Ovine, Ruminant, Other

Specimen

Name

Container

Minimum Amount

Brainstem

Sterile container


Uterine secretions

Sterile container


Conjunctival swab

Commercial culturette/Cotton tipped swab (See Collection Information)


Fetal liver, lung

Leakproof container

2 samples each


Placenta

Leakproof container

2-3 cotyledons


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit placenta (cotyledonary NOT amniotic).

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
FETUS/FETAL TISSUES - Double bag body if shipping through a courier service. Ship placenta in a separate leakproof, plastic bag.

Additional Information
Performed in conjunction with Aerobic Culture. This fee is in addition to the fee for Aerobic Culture.

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