Theileria orientalis/Anaplasma marginale PCR

Section: Molecular Diagnostics

Fee: $45.00

Turnaround Time: 1–2 business days

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Bovine, Other

Specimen

Name Container Minimum Amount
Whole BloodEDTA Purple Top Vacutainer 1-2 ml
SpleenSealed, Leakproof Container5 grams

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in an appropriate vacutainer tube, making sure to meet minimum quantity.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information

This test detects Anaplasma marginale and Theileria orientalis while distinguishing between genotypes chitose (type 1) and ikeda (type 2).