Bovine Respiratory PCR Panel

Section: Molecular Diagnostics

Fee: $85.00

Turnaround Time: 2–3 business days

Days Test Started: Tuesday, Thursday, Friday

Species: Bovine

Specimen

Name Container Minimum Amount
LungSealed, Leakproof Container8-10 grams
Deep nasal or nasopharyngeal swabLeakproof Tube
Tracheobronchial lavage fluidLeakproof Tube

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information

Available July 1st, 2022. The test includes Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza 3 Virus (PI3), Bovine Viral Diarrhea (BVD), Bovine Herpesvirus 1 (IBR), and Mycoplasma bovis PCR.
Swabs from the nares or cranial nasal cavity have limited ability to identify BRSV infection.