Porcine Circovirus Type 2 (PCV2) ORF 2 Sequencing
Section: Molecular Diagnostics
Fee: $130.00
Turnaround Time: 3 – 7 Business Days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Porcine
Specimen
Name
Container
Minimum Amount
Serum
Red top vacutainer tube
1-2 ml
Virus isolate
Oral Fluid
Leakproof container
5 - 10 ml
Lung/Lymph Node/Spleen
Leakproof container
Several samples of each
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
This test is performed following a positive PCV2 PCR test or virus isolation. Unless directed otherwise by submitter, the laboratory will sequence the sample with the lowest CT value, which should represent the sample with the most virus. PCV2 PCR Ct of 32 or lower is recommended for success of ORF 2 sequence.- Indiana Animal Disease Diagnostic Laboratory Home
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