Bovine Neonatal Diarrhea Screen PCR (BCV, BRV, Crypto, F5/K99, Salmonella)
Section: Molecular Diagnostics
Fee: $70.00
Turnaround Time: 1 – 2 business days
Days Test Started: Monday, Wednesday, Friday
Species: Bovine
Specimen
Name
Container
Minimum Amount
Feces
Sealed, Leakproof Container
10 - 20 grams
Fecal swab
Sealed, Leakproof Container
Intestine
Sealed, Leakproof Container
10 - 20 grams
Intestinal contents
Sealed, Leakproof container
10 - 20 grams
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leakproof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FECES - Ensure the container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
This PCR test detects Bovine Coronavirus, Bovine Rotavirus, Cryptosporidium, Fimbrial Antigen F5 (previously K99) of pathogenic E. coli strains, and Salmonella spp. in bovine neonatal feces.- Indiana Animal Disease Diagnostic Laboratory Home
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