College of Veterinary Medicine

Leptospira (7 serovars) MAT

Section: Bacteriology

Fee: $35.00

Turnaround Time: 1 – 4 business days

Days Test Started: Tuesday

Species: All Species

Specimen

Name

Container

Minimum Amount

Serum

Red top Vacutainer tube or equivalent

0.5 – 1 ml*


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. If multiple serology tests are requested on any one serum sample, the minimum amount is 1-1.5 ml. Refrigerate specimen(s) until shipped.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.

Additional Information
The seven serovars detected are L. autumnalis, L. bratislava, L. canicola, L grippotyphosa, L. hardjo, L. icterohemorrhagiae, L. pomona.

Contact lab in advance of large quantity submissions.

Purdue University College of Veterinary Medicine, 625 Harrison Street, West Lafayette, IN 47907, 765-494-7607

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