College of Veterinary Medicine

Escherichia coli (E. coli) F5 K99 PCR

Section: Molecular Diagnostics

Fee: $33.00

Turnaround Time: 3–8 business days

Days Test Started: Tuesday, Thursday

Species: Bovine, Porcine

Specimen

Name Container Minimum Amount
Bacterial isolateCulture plate or slant

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date. Please ensure the sample is a pure culture. Mixed cultures require additional isolation and identification at additional cost to the submitter.

Shipping Information

PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu. OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY

Additional Information

This test detects the presence of the gene encoding the fimbrial antigen F5, previously known as K99. F5 is an adhesin that allows pathogenic E. coli strains to adhere to intestinal cells and colonize the small intestine. The presence of F5 allows classification of the bacterial isolate as ETEC (Enterotoxigenic E. coli).
This test is also part of the Bovine Neonatal Diarrhea Screen PCR (Bovine Coronavirus, Bovine Rotavirus, Crypto, Salmonella, and F5/K99).

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