College of Veterinary Medicine

Caprine Arthritis Encephalitis Virus (CAE) ELISA (Lentivirus ELISA)

Section: Serology

Fee: $8.80 ($4.50 if >25 samples)

Turnaround Time: 2–3 business days (Samples must arrive by Wednesday)

Days Test Started: Thursday

Species: Caprine

Specimen

Name

Container

Minimum Amount

Serum

Red top Vacutainer tube or equivalent

0.5 – 1 ml*


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.

*If multiple serology tests are requested on any one serum sample, minimum amount is 1-1.5 ml.

Refrigerate specimen(s) until shipped.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection. If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Ship specimens overnight with an ice pack. DO NOT SHIP OVER WEEKENDS OR HOLIDAYS.

Additional Information
Contact lab for large quantity submissions.

ADDL West Lafayette location tests will be started on Thursday. Samples must be received by the Wednesday prior. Contact the lab for rush and/or off schedule testing for an additional fee.

As caprine serum degrades quickly on the clot, it is recommend to only send the serum. The preferred quality of serum for ELISA tests is a clear, translucent yellow sample. For more information, please see our resource on hemolysis

This test is also available as part of the Small Ruminant Serology Panel.

Purdue University College of Veterinary Medicine, 625 Harrison Street, West Lafayette, IN 47907, 765-494-7607

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