2025 Annual Report
A Letter from the Director, Dr. Kenitra Hendrix
The Indiana Animal Disease Diagnostic Laboratory (ADDL) experienced another busy year, underscoring the critical role we play in supporting agriculture and veterinary medicine. In 2025, highly pathogenic avian influenza virus (HPAI) was a major focus of our work, with the laboratory performing over 40,000 HPAI PCR tests to meet Indiana’s incident, surveillance, and movement testing needs.
Below, I highlight key accomplishments aligned with two of the ADDL’s strategic priorities: investing in our workforce and advancing our facilities and equipment.
We hope you will take time to explore this new accessible annual report format to learn more about our exceptional people and the impactful work they do every day. Thank you for your continued support and partnership as we move forward together.
Invest in a highly skilled workforce and provide a stimulating and rewarding work environment.
As always, our team delivered high-quality, timely diagnostic services to our customers. In 2025, we held two special events to recognize and thank our team for their dedication: a pancake breakfast prepared by myself and Dr. Bowen, Associate Director, and a surprise food truck event featuring ice cream and cookies. The food truck was generously sponsored by the Indiana State Poultry Association as a token of appreciation for our team’s extraordinary response to the HPAI outbreak.
We also continue to host semi-annual “Lunch and Learn” sessions to help staff better understand the broader impact of their work. If you or someone from your organization would be interested in speaking at one of these sessions - or sponsoring a lunch – we would welcome your involvement.
Invest in state-of-the-art equipment and facilities.
Phase one of the necropsy renovation project was completed with the installation of a new incinerator, improving both efficiency and biosafety in material disposal. Phase two, which includes replacement of the walk-in cooler, began in December and is expected to be completed in late spring 2026.
In addition, we acquired a whole-slide scanner and digital pathology platform, transitioning diagnostic microscopy to a fully digital environment. This advancement supports the integration of advanced image analysis and artificial intelligence (AI)–assisted diagnostics, enables rapid global specialist consultation, enhances image sharing for clients and trainees, and promotes more efficient workflows and research innovation.
Diagnostic Insights
Accessioning Counts
Annual diagnostic accession (case) volumes processed by the Reed ADDL and Heeke ADDL from 2023 through 2025.
| Location | 2023 | 2024 | 2025 |
|---|---|---|---|
| Reed ADDL | 28,131 | 31,406 | 39,978 |
| Heeke ADDL | 2,188 | 2,373 | 2,177 |
| Total | 30,319 | 33,779 | 42,155 |
Test Counts
Annual diagnostic test volumes processed by the Reed ADDL and Heeke ADDL from 2023 through 2025.
| Location | 2023 | 2024 | 2025 |
|---|---|---|---|
| Reed ADDL | 130,577 | 142,266 | 176,473 |
| Heeke ADDL | 49,322 | 56,314 | 52,309 |
| Total | 179,899 | 198,580 | 228,782 |
Top Three Tests - By Species
Summary of diagnostic accessions submitted to the ADDL (Reed and Heeke combined) by animal species and year (2023-2025), including total accession counts and the three most frequently performed tests for each species.
| Species | Year | Total Accessions | Top Test 1 ( n) | Top Test 2 ( n) | Top Test 3 ( n) |
|---|---|---|---|---|---|
| Aquatic | 2023 | 65 | Virus Isolation (50) | Aerobic Culture (27) | Renibacterium salmoninarum-FA (17) |
| Aquatic | 2024 | 64 | Virus Isolation (47) | Aerobic Culture (23) | Renibacterium salmoninarum-FA (17) |
| Aquatic | 2025 | 69 | Virus Isolation (49) | Aerobic Culture (32) | Necropsy (22) |
| Avian | 2023 | 6,523 | Avian Influenza (3,099) | Turkey Coronavirus - PCR (1,051) | Necropsy (618) |
| Avian | 2024 | 7,983 | Avian Influenza (3,762) | Avian Metapneumovirus - PCR (1,168) | Turkey Coronavirus - PCR (901) |
| Avian | 2025 | 16,699 | Avian Influenza (13,480) | Avian Metapneumovirus - PCR (1,428) | Turkey Coronavirus - PCR (970) |
| Bovine | 2023 | 1,705 | BVD-PI-ELISA & PCR (682) | Johne's - ELISA (266) | Necropsy (186) |
| Bovine | 2024 | 1,895 | BVD-PI-ELISA & PCR (723) | Johne's - ELISA (285) | Necropsy (222) |
| Bovine | 2025 | 4,695 | Avian Influenza (2,796) | Bovine Influenza A - ELISA (777) | BVD-PI-ELISA & PCR (644) |
| Camelid | 2023 | 105 | Fecal Flotation (77) | Aerobic Culture (19) | Necropsy (12) |
| Camelid | 2024 | 103 | Fecal Flotation (108) | Necropsy (19) | Aerobic Culture (12) |
| Camelid | 2025 | 111 | Fecal Flotation (97) | Necropsy (18) | Mycoplasma Haemollamica (7) |
| Canine | 2023 | 8,583 | Surgical Histopathology (4,335) | Aerobic Culture (1,734) | Neuroaxonal Dystrophy - PCR (1,201) |
| Canine | 2024 | 10,562 | Surgical Histopathology (4,167) | Neuroaxonal Dystrophy - PCR (3,620) | Aerobic Culture (1,421) |
| Canine | 2025 | 7,301 | Surgical Histopathology (3,973) | Aerobic Culture (1,398) | Aerobic Sensitivity (590) |
| Caprine | 2023 | 843 | Fecal Flotation (355) | Small Ruminant Lentiviruses-ELISA (202) | C. pseudotuberculosis - ELISA (163) |
| Caprine | 2024 | 971 | Fecal Flotation (343) | Small Ruminant Lentiviruses-ELISA (267) | Johne's ELISA (245) |
| Caprine | 2025 | 902 | Small Ruminant Lentiviruses-ELISA (253) | Fecal Flotation (251) | Johne's ELISA (217) |
| Cervid | 2023 | 1,136 | CWD - Histology (963) | Brucella abortus (91) | Necropsy (35) |
| Cervid | 2024 | 1,304 | CWD - ELISA & Histology (1,208) | Necropsy (50) | Fecal Flotation (48) |
| Cervid | 2025 | 1,196 | CWD - ELISA & Histology (1,081) | Necropsy (39) | Brucella abortus (36) |
| Equine | 2023 | 6,701 | EIA - ELISA & AGID (4,756) | Fecal Flotation (751) | Aerobic Culture (355) |
| Equine | 2024 | 6,201 | EIA - ELISA & AGID (4,369) | Fecal Flotation (798) | Salmonella - PCR (200) |
| Equine | 2025 | 6,527 | EIA - ELISA & AGID (4,646) | Fecal Flotation (965) | Aerobic Culture (229) |
| Feline | 2023 | 1,516 | Surgical Histopathology (598) | Aerobic Culture (565) | Necropsy (171) |
| Feline | 2024 | 1,507 | Surgical Histopathology (628) | Aerobic Culture (501) | Necropsy (170) |
| Feline | 2025 | 1,538 | Surgical Histopathology (596) | Aerobic Culture (508) | Necropsy (172) |
| Ovine | 2023 | 227 | Fecal Flotation (88) | Necropsy (43) | Aerobic Culture (45) |
| Ovine | 2024 | 271 | Fecal Flotation (101) | Necropsy (53) | Aerobic Culture (52) |
| Ovine | 2025 | 267 | Fecal Flotation (68) | C. pseudotuberculosis - ELISA (40) | Johne's ELISA (39) |
| Porcine | 2023 | 2,057 | Pseudorabies - ELISA (716) | Brucella abortus (713) | PRRS - PCR (698) |
| Porcine | 2024 | 2,035 | PRRS-PCR (902) | Brucella abortus (620) | Pseudorabies - ELISA (619) |
| Porcine | 2025 | 1,889 | PRRS-PCR (859) | Pseudorabies - ELISA (569) | Brucella abortus (547) |
| Lab Animal | 2023 | 88 | Aerobic Culture (14) | Anaerobic Culture (14) | Fungal Culture (8) |
| Lab Animal | 2024 | 103 | Aerobic Culture (15) | Anaerobic Culture (15) | Fungal Culture (11) |
| Lab Animal | 2025 | 91 | Fungal Culture (8) | Aerobic Culture (6) | Anaerobic Culture (6) |
| Miscellaneous | 2023 | 397 | Fecal Flotation (159) | Necropsy (64) | Rabies (56) |
| Miscellaneous | 2024 | 436 | Fecal Flotation (138) | Rabies (71) | Necropsy (59) |
| Miscellaneous | 2025 | 460 | Fecal Flotation (134) | Rabies (83) | Necropsy (55) |
Top Three Tests - By Laboratory Section
Summary of diagnostic accessions submitted to the ADDL by laboratory section and year (2023-2025), including total accession counts and the three most frequently requested tests for each department.
| Department | Year | Total Accessions | Top Test 1 ( n) | Top Test 2 ( n) | Top Test 3 ( n) |
|---|---|---|---|---|---|
| Bacteriology | 2023 | 5,176 | Aerobic Culture (4,347) | Aerobic Sensitivity (1,484) | Anaerobic Culture (848) |
| Bacteriology | 2024 | 4,672 | Aerobic Culture (3,858) | Aerobic Sensitivity (1,324) | Anaerobic Culture (820) |
| Bacteriology | 2025 | 4,093 | Aerobic Culture (3,366) | Aerobic Sensitivity (1,087) | Anaerobic Culture (724) |
| Histology | 2023 | 5,648 | Surgical Histopathology [<5cm] (3,802) | Surgical Histopathology [5-8 cm] (817) | Surgical Histopathology [>8cm] (341) |
| Histology | 2024 | 5,599 | Surgical Histopathology [<5cm] (3,726) | Surgical Histopathology [5-8 cm] (763) | Surgical Histopathology [>8cm] (334) |
| Histology | 2024 | 5,277 | Surgical Histopathology [<5cm] (3,481) | Surgical Histopathology [5-8 cm] (690) | Surgical Histopathology [>8cm] (356) |
| Molecular Diagnostics | 2023 | 8,774 | Avian Influenza - PCR (3,206) | Neuroaxonal Dystrophy -PCR (1,201) | Turkey Coronavirus - PCR (1,051) |
| Molecular Diagnostics | 2024 | 10,285 | Avian Influenza - PCR (3,865) | Avian Metapneumovirus -PCR (1,168) | Newcastle -PCR (1,040) |
| Molecular Diagnostics | 2025 | 21,588 | Avian Influenza - PCR (16,382) | Avian Metapneumovirus -PCR (1,429) | Turkey Coronavirus - PCR (970) |
| Parasitology | 2023 | 2,338 | Fecal Flotation - Qualitative (1201) | Fecal Flotation - Quantitative (1098) | Giardia/Crypto Panel (124) |
| Parasitology | 2024 | 2,286 | Fecal Flotation - Qualitative (1199) | Fecal Flotation - Quantitative (1096) | Giardia/Crypto Panel (91) |
| Parasitology | 2025 | 2,515 | Fecal Flotation - Qualitative (1265) | Fecal Flotation - Quantitative (1226) | Parasite ID (113) |
| Pathology | 2023 | 7,483 | Surgical Histopathology - Reports (5,266) | Necropsy - Reports (1,894) | IHC (255) |
| Pathology | 2024 | 7,255 | Surgical Histopathology - Reports (5,081) | Necropsy - Reports (1,948) | IHC (263) |
| Pathology | 2025 | 6,840 | Surgical Histopathology - Reports (4,793) | Necropsy - Reports (1,889) | IHC (229) |
| Serology | 2023 | 6,714 | EIA - ELISA & AGID (4,766) | Brucella abortus (939) | Pseudorabies - ELISA (716) |
| Serology | 2024 | 6,272 | EIA - ELISA & AGID (4,399) | Brucella abortus (755) | Pseudorabies - ELISA (619) |
| Serology | 2025 | 7,257 | EIA - ELISA & AGID (4,672) | Bovine Influenza - ELISA (777) | Brucella abortus (690) |
| TSE | 2023 | 1,030 | CWD - Histology (963) | Scrapie (65) | |
| TSE | 2024 | 1,243 | CWD - Histology (1,167) | Scrapie (66) | |
| TSE | 2025 | 1,102 | CWD - Histology (1,048) | Scrapie (54) | |
| Toxicology | 2023 | 392 | Trace Minerals - Serum (51) | Bone Marrow Fat (43) | Anticoagulant (39) |
| Toxicology | 2024 | 410 | Trace Minerals - Serum (57) | Bone Marrow Fat (54) | Vitamin E (43) |
| Toxicology | 2025 | 398 | Trace Minerals - Serum (53) | Nitrate-Qualitative (42) | Bone Marrow Fat (39) |
| Virology | 2023 | 854 | BVD - ACE (449) | Brucella canis - IFA (130) | Virus Isolation (51) |
| Virology | 2024 | 680 | BVD - ACE (403) | Brucella canis - IFA (91) | Virus Isolation (48) |
| Virology | 2025 | 764 | BVD - ACE (362) | Canine Distemper Virus-IFA (152) | Brucella canis - IFA (92) |
USA Distribution and Top Five
State-level distribution of diagnostic samples submitted to the ADDL in 2025, with total accession counts displayed for the five highest submitting states. The five highest submitting states are labeled with totals: Indiana (36,291), Ohio (2,055), Michigan (1,084), Illinois (624), and North Carolina (474). Most other states show lower submission ranges from 1 to 100.
Indiana Distribution
Statewide distribution of diagnostic accessions submitted to the ADDL by county in Indiana during 2025, based on accessions with available ZIP code or city information. The five highest submitting counties are Davies (1,000 - 2,000), Dubois (1,000 - 2,000), Elkhart (>2,000), and Tippecanoe (>2,000). The accession range for the other Indiana counties is <50 - 1,000.
Case Distribution - By Laboratory Section
Distribution of diagnostic accessions processed by ADDL laboratory sections in 2025 including multi-section cases. Molecular Diagnostics represents the largest share at 43.0%, followed by Serology (14.4%), Pathology (13.6%), Histology (10.5%), and Bacteriology (8.0%). Smaller sections include Parasitology (4.9%), TSE (2.7%), Virology (1.5%), Toxicology (0.8%), Canine Genetics (0.7%), and Avian Diagnostics (0.3%).
Case Distribution - By Species
Distribution of diagnostic accessions submitted to the ADDL by animal species in 2025. Avian cases make up the largest share at 39.6%, followed by Canine at 17.3%, Equine at 15.5%, and Bovine at 11.1%. Smaller proportions include Porcine (4.5%), Feline (3.7%), Cervid (2.8%), Caprine (2.1%), Miscellaneous (1.1%), Non-animal (0.8%), Ovine (0.6%), Camelid (0.3%), Lab Animal (0.2%), Aquatic (0.2%), and Unknown (0.1%).
Revenue Sources
| Type of Revenue | FY 2023 | FY 2024 | FY 2025 |
|---|---|---|---|
| Fees | $2,772,162.73 | $3,769,892.10 | $4,674,878.00 |
| Grants | $484,295.51 | $1,223,456.19 | $306,336.00 |
| State Appropriations | $3,711,516.00 | $5,000,000.00 | $5,000,000.00 |
| Total | $6,967,974.24 | $9,993,348.29 | $9,981,214.00 |
Staff Highlights
Promotions
Dr. Kenitra Hendrix
Clinical Professor
Dr. Mario Sola
Clinical Associate Professor
Dr. Grant Burcham
Clinical Associate Professor
Awards
Dr. Abigail Cox
2024 Boehringer Ingelheim Best Mentor Award (presented in 2025)
Dr. Mario F. Sola
2025 PVM Award for Excellence in Teaching
Dr. Krysten Schmidt
Davis-Thompson Foundation Scholarship Award
Cristy Rice
Bravo Award
Megan Lintner
Bravo Award
Angie Chan
PVM Outstanding Staff Award
Dr. Abigail English
2025 H. Leon Thacker Award Recipient
Years of Service
Debra Lubelski
35 Years
Art Armstrong
25 Years
Stephanie Martinez-Morales
10 Years
Margie Veverka
10 Years
Staff
West Lafayette Staff
Heeke Staff
ADDL Advisory Committee
The ADDL Advisory Committee serves the critical role of representing the interests of various sectors to the ADDL leadership.
- John Baugh, College of Agriculture
- Michelle Benavidez Westrich, IN Department of Natural Resources
- Paul Brennan, Poultry
- Jenni Browning, American Dairy Association
- Dr. Angela Hines, USDA/APHIS
- Dr. Jake Jensen, Equine
- Becky Joniskan, Indiana State Poultry Association
- Dr. Greg Kurtz, Dairy, Beef Cattle
- Dr. Ellen Lowery, Purdue Veterinary Hospital
- Dean Bret Marsh, Purdue College of Veterinary Medicine
- Megan Ritter, Indiana Farm Bureau
- Dr. Sandra San Miguel, Associate Dean for Engagement, PVM
- Dr. Joel Sparks, Porcine
- Josh Trenary, Indiana Pork Producers
Resources
Submission Forms
ADDL submission forms are routinely updated with the current test offerings. Please visit Submission Forms page of the website to ensure you are up-to-date.
How You Can Help
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Publications
- Chandra S, Cezar G, Rupasinghe K, Magalhães E, Silva GS, Almeida M, Crim B, Burrough E, Gauger P, Madson D, Thomas J, Zeller M, Zhang J, Main R, Rovira A, Thurn M, Lages P, Corzo C, Sturos M, VanderWaal K, Naikare H, Matias-Ferreyra F, McGaughey R, Retallick J, McReynolds S, Gebhardt J, Pillatzki A, Greseth J, Kersey D, Clement T, Christopher-Hennings J, Thompson B, Perkins J, Prarat M, Summers D, Bowen C, Boyle J, Hendrix K, Lyons J, Werling K, Arruda AG, Schwartz M, Yeske P, Murray D, Mason B, Schneider P, Copeland S, Dufresne L, Boykin D, Fruge C, Hollis W, Robbins R, Petznick T, Kuecker K, Glowzenski L, Niederwerder M, Huang X, Linhares DCL, Trevisan G. Harnessing sequencing data for porcine reproductive and respiratory syndrome virus (PRRSV): tracking genetic evolution dynamics and emerging sequences in US swine industry. Front Vet Sci. 2025 Mar 6;12:1571020. https://doi.org/10.3389/fvets.2025.1571020
- Moraes DCA, Cezar GA, Magalhães ES, Nicolino RR, Rupasinghe K, Chandra S, Silva GS, Almeida MN, Crim B, Burrough ER, Gauger PC, Madson D, Thomas J, Zeller MA, Main R, Thurn M, Lages P, Corzo CA, Sturos M, Naikare H, McGaughey R, Matias Ferreyra F, Retallick J, Gebhardt J, McReynolds S, Greseth J, Kersey D, Clement T, Pillatzki A, Christopher-Hennings J, Thompson BS, Prarat M, Summers D, Bowen C, Boyle J, Hendrix K, Lyons J, Werling K, Arruda AG, Schwartz M, Yeske P, Murray D, Mason B, Schneider P, Copeland S, Dufresne L, Boykin D, Fruge C, Hollis W, Robbins RC, Petznick T, Kuecker K, Glowzenski L, Niederwerder M, Linhares DCL, Trevisan G. Macroepidemiological trends of Influenza A virus detection through reverse transcription real-time polymerase chain reaction (RT-rtPCR) in porcine samples in the United States over the last 20 years. Front Vet Sci. 2025 Apr 24;12:1572237. https://doi.org/10.3389/fvets.2025.1572237
- Habbani SF, Dowlatshahi S, Lichti N, Broman M, Tecle L, Bolton S, Flowers L, Guerrero-Preston R, Linnes JC, Mohammed SI. Protein Biomarkers Enable Sensitive and Specific Cervical Intraepithelial Neoplasia (CIN) II/III+ Detection: One Step Closer to Universal Cervical Cancer Screening. Cancers (Basel). 2025 May 24;17(11):1763. https://doi.org/10.3390/cancers17111763
- Shourya Verma, Aditya Malusare, Mengbo Wang, Luopin Wang, Arpan Mahapatra, Abigail English, Abigail Cox, Meaghan Broman, Simone DeBrot, Grant Burcham, Deborah Knapp, Deepika Dhawan, Mario Sola, Vaneet Aggarwal, Ananth Grama, Nadi a Atallah Lanman AnnotateAnyCell: Open-Source AI Framework for Efficient Annotation in Digital Pathology bioRxiv 2025.11.02.686114; doi: https://doi.org/10.1101/2025.11.02.686114
- Broman MM, Lanman NA, Vickman RE, Cresswell GM, Kothandaraman H, Kolliegbo A, Paez JSP, Glaser AP, Helfand BT, Henry G, Strand DW, Franco OE, Hayward SW, Ratliff TL. Immune cell single-cell RNA sequencing analyses link an age-associated T cell subset to symptomatic benign prostatic hyperplasia. Front Immunol. 2025 Jul 7;16:1585446. doi: 10.3389/fimmu.2025.1585446. eCollection 2025.PMID: 40692782. https://pubmed.ncbi.nlm.nih.gov/40692782/
- Dudley AM, Wahl ML, Humberg LA, Kluever BM, Burcham GN, Zollner PA. Spatial responses of black vultures to resource pulses during white-tailed deer hunting season. Wildl Soc Bull. e1584. April 2025. https://www.researchgate.net/publication/390470246_Spatial_responses_of_black_vultures_to_resource_pulses_during_white-tailed_deer_hunting_seasons
- McWherter B, Ma Z, Wahl M, Zollner P, Humberg L, Kluever B, Burcham GN. Drivers of agricultural producers' tolerance towards less-charismatic avian species. Biological Conservation. Vol 301. Article 110884. January 2025. https://www.sciencedirect.com/science/article/pii/S0006320724004464
- Wilkes RP, Chan A, Elshafie N, Robbins K. Development and validation of a real-time PCR assay for Cochlosoma anatis in turkeys. J Vet Diagn Invest. 2025 Nov;37(6):952-955. https://doi.org/10.1177/10406387251364260
- Beli E, Yan Y, Moldovan L, Lydic TA, Krishman P, Tersey SA, Duan Y, Salazar TE, Dominguez JM, Nguyen DV, Cox A, Li Calzi S, Beam C, Mirmira RG, Evans-Molina C, Busik JV, Grant MB. Reshaping lipid metabolism with long-term alternate day feeding in type 2 diabetes mice. NPJ Metab Health Dis. 2025;3(1):3. https://doi.org/10.1038/s44324-024-00039-w
- Boycott C, Beetch M, Lubecka-Gajewska K, Ramsey BS, Torregrosa-Allen S, Elzey BD, Cox A, Lanman NA, de Conti A, Li M, Ferruzzi M, Pogribny I, Stefanska B. AMPK-Dependent Epigenetic Regulation of Metabolism Mediates the Anti-Cancer Action of Pterostilbene in Hepatocellular Carcinoma. Mol Nutr Food Res. 2025 Dec;69(23):e70217. https://doi.org/10.1002/mnfr.70217
- Samaddar M, Sun C, Gallina NLF, Irizarry-Tardi N, Liu D, Tenguria S, Drolia R, Jain A, Kihara D, Jiang W, Kim KH, Cox A, Ristroph KD, Knipp GT, Noinaj N, Bhunia AK. LAP-Hsp60 complex modulates epithelial tight junction barrier. Res Sq [Preprint]. 2025 May 14:rs.3.rs-6474377. https://doi.org/10.21203/rs.3.rs-6474377/v1
- Rodgers B, Cannes do Nascimento N, Cox A, Bailey TW, Sivasankar MP, Schaser AJ. Molecular changes, histopathology, and ultrasonic vocalization acoustic profiles of systemically dehydrated rats. PLoS One. 2025 Apr 22;20(4):e0322187. https://doi.org/10.1371/journal.pone.0322187
- Frankovich A, Miller C, Barnhart K, Manickam B, Vemireddi V, Wallace S, Cox A. Letter to the Editor: Empowering pathologists with a tool to report pathology errors in publications. Vet Pathol. 2025 Sep;62(5):806-807. https://doi.org/10.1177/03009858251349110
- Manikantan V, Pires Dos Santos A, Dangoudoubiyam S. The expanding role of microRNAs in the biology and control of veterinary parasitic nematodes. Front Vet Sci. 2025 Sep 19;12:1668932. https://doi.org/10.3389/fvets.2025.1668932
- Sanders TL, Sobotyk C, Jimenez Castro PD, Abdu A, Baade J, Borst M, Dangoudoubiyam S, Delcambre BA, Gruntmeir JM, Lee A, Leutenegger C, Lozoya C, Murphy G, Pulaski C, Schaefer J, Vatta A, Walden HDS, Lejeune M, Verocai GG. Molecular characterization of Spirometra isolates across the USA. Parasitology. 2025 Apr;152(5):477-486. https://doi.org/10.1017/S003118202500054X
- Jegatheesan A, Micciche M, Ngo J, Bradley PJ, Howe DK, Dangoudoubiyam S. Characterization of SnROP9, a rhoptry protein homologue of Sarcocystis neurona that is expressed in lifecycle stages lacking rhoptry organelles. Int J Parasitol. 2025 May;55(6):327-337. https://doi.org/10.1016/j.ijpara.2025.02.001
- Page LK, Loiacono A, Edmunds S, Black B, Dangoudoubiyam S, Anchor C. Sensitivity of Methods to Determine Presence of Baylisascaris procyonis Eggs in Raccoon (Procyon lotor) Feces. J Wildl Dis. 2025 Apr 1;61(2):483-487. https://doi.org/10.7589/JWD-D-24-00090
- Bilhalva LC, Serpa PBS, Strandberg NJ, Dangoudoubiyam S, Thompson CA, Santos AP. What Is Your Diagnosis? Esophageal and Gastric Washes in a Pet Moellendorff's Rat Snake. Vet Clin Pathol. 2025 Nov 3. https://doi.org/10.1111/vcp.70073
- Elshafie, N.O.; Kattoor, J.J.; Kelly, J.; Wilkes, R.P. MinION Adapted tNGS Panel for Carnivore Pathogens Including SARS-CoV-2. Pathogens2026, 15, 23. https://www.mdpi.com/2076-0817/15/1/23
- Kattoor JJ, Anis E, Elshafie NO, Wilkes RP. A multiplex targeted NGS panel for identifying pathogens in canine neurological and reproductive diseases. Front Cell Infect Microbiol. 2025 Aug 1;15:1591145. https://www.mdpi.com/2076-0817/15/1/23. PMID: 40822582; PMCID: PMC12355606.
- Elshafie NO, Wilkes RP. Oxford Nanopore Targeted Sequencing Assay for Flongle Flow Cells. protocols.io https://www.protocols.io/view/oxford-nanopore-targeted-sequencing-assay-for-flon-3byl46ewrgo5/v2
- Cole SD, Paul NC, Hendrix K, Lawhon SD, Rankin SC, Diaz-Campos D, Maboni G, Burbick CR, Surendran-Nair M, Daniels JB, Tewari D, Rudd JM, Sobotyk C, LeCuyer TE. Collaboration with the clinical microbiology laboratory optimizes diagnosis of dog and cat infections: recommendations from the American College of Veterinary Microbiologists. J Am Vet Med Assoc. 2025 Mar 25;263(S1):S88-S97. https://doi.org/10.2460/javma.24.12.0776
- Systemic granulomatosis associated with hairy vetch toxicosis in a cattle herd.
Pillai VV, Sola MF, Hooser SB. Res Vet Sci. 2025 Nov;196:105905. doi: 10.1016/j.rvsc.2025.105905. Epub 2025 Sep 22. PMID: 40997739 https://pubmed.ncbi.nlm.nih.gov/40997739/
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Hypoxia-inducible factor 1α expression does not differ between canine urothelial carcinoma and normal urinary bladder tissue.Gibbs NH, Sola MF, Dhawan D, Knapp D, Foster JD, Kendall A, Moore GE, Woolcock A.Am J Vet Res. 2025 Apr 28;86(7):ajvr.24.12.0391. doi: 10.2460/ajvr.24.12.0391. Print 2025 Jul 1. PMID: 40294640 https://pubmed.ncbi.nlm.nih.gov/40294640/
- Characterisation of Gene Expression in Canine Invasive Urothelial Carcinoma Using a NanoString-Based Urine Assay. Kuerbitz EM, Dhawan D, Utturkar S, Sola MF, Enstrom AW, Fourez LM, Knapp DW. Vet Comp Oncol. 2025 Jun;23(2):205-215. doi: 10.1111/vco.13046. Epub 2025 Mar 19. PMID: 40104990 https://pubmed.ncbi.nlm.nih.gov/40104990/
- Alhashimi M, Sayedahmed EE, Elkashif A, Chothe SK, Wang WC, Murala MST, Gairola V, Wakamatsu N, Gontu A, Ramasamy S, LaBella L, Jakka P, Nair MS, Nissly RH, Kuchipudi SV, Mittal SK. A multi-antigen-based SARS-CoV-2 vaccine provides higher immune responses and protection against SARS-CoV-2 variants. NPJ Vaccines. 2025 Jul 19;10(1):159. https://doi.org/10.1038/s41541-025-01198-7
- Reuter A, Diaz MPBR, Wakamatsu N, McManamey A. Lameness, generalized illness, and sudden death in a 2-year-old Doberman Pinscher. J Am Vet Med Assoc. 2025 Sep 24;264(1):108-111. https://doi.org/10.2460/javma.25.07.0460
- Penticoff HB, Baden R, Speer B, Wakamatsu N, Tully TN. Five cases of psittacine proventricular and ventricular tumors. Vet Research Case Reports. 2025;e70168. doi.org/10.1002/vrc2.70168. https://bvajournals.onlinelibrary.wiley.com/doi/10.1002/vrc2.70168
Abstracts
- Burcham GN. A novel caprine astrovirus detected in conjunction with necrotizing enterocolitis in goat kids. Abstract 4382093. Pathology Scientific Session. AAVLD Annual Meeting. Denver, CO, November, 2025
- Elshafie N. Development of a Targeted Next-Generation Sequencing Panel for Common and Emerging Swine Pathogens, AAVLD, Aurora, CO
- Elshafie N. Gene Expression Profiling of Canine Diffuse Large B-cell Lymphoma: Insights from RNA Sequencing, AACR, Chicago, IL
- Elshafie N. Adapting a Targeted NGS Panel for Carnivore Pathogen Detection, Including SARS-CoV-2, to the Oxford Nanopore MinION (Diagnostic Testing), CRWAD, Chicago, IL
Outreach
- Burcham GN. Asian longhorned tick and bovine theileriosis in Indiana. Southern Indiana Beef Meetings by Zoetis, Indiana Beef Cattle Association and Purdue University Extension. Southern Hills Church, Salem, IN. September 23, 2025
- Burcham GN. Asian longhorned tick and bovine theileriosis in Indiana. Southern Indiana Beef Meetings by Zoetis, Indiana Beef Cattle Association and Purdue University Extension. Southern Indiana Purdue Agricultural Center. Dubois, IN. September 22, 2025
- Burcham GN. Animal Disease Diagnostic Laboratory: Overview. Purdue Extension Monthly Hot Topics. Webinar. July 17, 2025
- Burcham GN. Bird and Grassland Interactions. Pasture Ecology Workshop. Southern Indiana Purdue Agricultural Center (SIPAC), Dubois, IN. June 18, 2025
- Burcham GN. Emerging Vector-borne Veterinary Diseases in Indiana. Indiana Vector Control Association Annual Meeting. Clifty Falls State Park, Madison, IN. March 2025
- Burcham GN. Salmonella, Mycoplasma, and AI. Indiana State Poultry Association Blood Testing School. Jasper, IN. March 2025
- Burcham GN. Common infectious diseases of backyard poultry, Indiana State Poultry Association Blood Testing School. Jasper, IN. March 2025
