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College of Veterinary Medicine

Nelli Lab Publications

Our publications highlight the findings, collaborations, and scientific contributions that have emerged from our research. Explore peer-reviewed articles, conference papers, and other scholarly works produced by our lab.

  • Nelli, R. K., Harm, T. A., Arruda, B., Siepker, C., Fasina, O., Groeltz-Thrush, J. M., Baker, A., Phillips, R., Jones, B., Espina, V., Seger, H., Kumar, M., Hassan, Q. U., Brixius, G., Caceres, C. J., Carnaccini, S., Gimenez-Lirola, L., Plummer, P. J. & Bell, T. M. Exploring influenza A virus receptor distribution in the lactating mammary gland of domesticated livestock and in human breast tissue Journal of Dairy Science 109(2) 1891-1906. https://doi.org/10.3168/jds.2025-26950
  • Twu, N., Teoh, Y. C., Tan, C. P. H., Danudoro, R., Cheng, X., Espin-Palazon, R., Bell, T., Giménez-Lirola, L., Chowdhury, R. & Nelli, R. K. Spatial mapping of influenza and coronavirus receptors in the respiratory and intestinal tract epithelium of beef cattle using advanced PixF image analysis Scientific Reports 15(1) https://doi.org/10.1038/s41598-025-28429-0
  • Gorden, P. J., Magstadt, D. R., Baker, A. L., Arruda, B. L., Bell, T. M. & Nelli, R. K. Viral Mastitis Associated with Influenza A in Dairy Cattle Veterinary Clinics of North America: Food Animal Practice 41(2) 271-283. https://doi.org/10.1016/j.cvfa.2025.02.010
  • Yen, L., Nelli, R. K., Twu, N., Mora-Díaz, J. C., Castillo, G., Sitthicharoenchai, P. & Giménez-Lirola, L. G. Development and characterization of segment-specific enteroids from the pig small intestine in Matrigel and transwell inserts: insights into susceptibility to porcine epidemic diarrhea Virus Frontiers in Immunology 15 https://doi.org/10.3389/fimmu.2024.1451154
  • Giménez-Lirola, L. G., Cauwels, B., Mora-Díaz, J. C., Magtoto, R., Hernández, J., Cordero-Ortiz, M., Nelli, R. K., Gorden, P. J., Magstadt, D. R. & Baum, D. H. Detection and Monitoring of Highly Pathogenic Influenza A Virus 2.3.4.4b Outbreak in Dairy Cattle in the United States Viruses 16(9) 1376. https://doi.org/10.3390/v16091376
  • Nelli, R. K., Harm, T. A., Siepker, C., Groeltz-Thrush, J. M., Jones, B., Twu, N., Nenninger, A. S., Magstadt, D. R., Burrough, E. R., Piñeyro, P. E., Mainenti, M., Carnaccini, S., Plummer, P. J. & Bell, T. M. Sialic Acid Receptor Specificity in Mammary Gland of Dairy Cattle Infected with Highly Pathogenic Avian Influenza A(H5N1) Virus Emerging Infectious Diseases 30(7) https://doi.org/10.3201/eid3007.240689
  • Davila, K. M. S., Nelli, R. K., Mora-Díaz, J. C., Sang, Y., Miller, L. C. & Giménez-Lirola, L. G. Transcriptome Analysis in Air–Liquid Interface Porcine Respiratory Epithelial Cell Cultures Reveals That the Betacoronavirus Porcine Encephalomyelitis Hemagglutinating Virus Induces a Robust Interferon Response to Infection Viruses 16(6) 939. https://doi.org/10.3390/v16060939
  • Nelli, R., Sarlo Davila, K., Phadke, K., Ruden, R., Bellaire, B., Sang, Y., Giménez-Lirola, L. & Miller, L. SARS-CoV-2 WA1 induces distinct immune transcriptome profiles in human and deer primary respiratory epithelial cells The Journal of Immunology 212(1_Supplement) 0124_4110-0124_4110. https://doi.org/10.4049/jimmunol.212.supp.0124.4110
  • Sarlo Davila, K. M., Nelli, R. K., Phadke, K. S., Ruden, R. M., Sang, Y., Bellaire, B. H., Gimenez-Lirola, L. G. & Miller, L. C. How do deer respiratory epithelial cells weather the initial storm of SARS-CoV-2 WA1/2020 strain? Microbiology Spectrum 12(2) https://doi.org/10.1128/spectrum.02524-23
  • Davila, K. M. S., Nelli, R. K., Phadke, K. S., Ruden, R. M., Yongming, S., Bellaire, B. H., Gimenez-Lirola, L. G. & Miller, L. C. How Do Deer Respiratory Epithelial Cells Weather The Initial Storm of SARS-CoV-2? https://doi.org/10.1101/2023.04.24.538130
  • Castillo, G., Mora-Díaz, J. C., Breuer, M., Singh, P., Nelli, R. K. & Giménez-Lirola, L. G. Molecular mechanisms of human coronavirus NL63 infection and replication Virus Research 327 199078. https://doi.org/10.1016/j.virusres.2023.199078
  • Castillo, G., Nelli, R. K., Phadke, K. S., Bravo-Parra, M., Mora-Díaz, J. C., Bellaire, B. H. & Giménez-Lirola, L. G. SARS-CoV-2 Is More Efficient than HCoV-NL63 in Infecting a Small Subpopulation of ACE2+ Human Respiratory Epithelial Cells Viruses 15(3) 736. https://doi.org/10.3390/v15030736
  • Yen, L., Mora-Díaz, J. C., Rauh, R., Nelson, W., Castillo, G., Ye, F., Zhang, J., Baum, D., Zimmerman, J., Nelli, R. & Giménez-Lirola, L. Characterization of the Subclinical Infection of Porcine Deltacoronavirus in Grower Pigs under Experimental Conditions Viruses 14(10) 2144. https://doi.org/10.3390/v14102144
  • Nelli, R., Roth, J. & Gimenez-Lirola, L. Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract Veterinary Sciences 9(9) 500. https://doi.org/10.3390/vetsci9090500
  • Castillo, G., Mora-Díaz, J. C., Nelli, R. K. & Giménez-Lirola, L. G. Human Air-Liquid-Interface Organotypic Airway Cultures Express Significantly More ACE2 Receptor Protein and Are More Susceptible to HCoV-NL63 Infection than Monolayer Cultures of Primary Respiratory Epithelial Cells Microbiology Spectrum 10(4) https://doi.org/10.1128/spectrum.01639-22
  • Yen, L., Magtoto, R., Mora-Díaz, J. C., Carrillo-Ávila, J. A., Zhang, J., Cheng, T., Magtoto, P., Nelli, R. K., Baum, D. H., Zimmerman, J. J. & Giménez-Lirola, L. G. The N-terminal Subunit of the Porcine Deltacoronavirus Spike Recombinant Protein (S1) Does Not Serologically Cross-react with Other Porcine Coronaviruses Pathogens 11(8) 910. https://doi.org/10.3390/pathogens11080910
  • Willgert, K., Didelot, X., Surendran-Nair, M., Kuchipudi, S. V., Ruden, R. M., Yon, M., Nissly, R. H., Vandegrift, K. J., Nelli, R. K., Li, L., Jayarao, B. M., Levine, N., Olsen, R. J., Davis, J. J., Musser, J. M., Hudson, P. J., Kapur, V. & Conlan, A. J. K. Transmission history of SARS-CoV-2 in humans and white-tailed deer Scientific Reports 12(1) https://doi.org/10.1038/s41598-022-16071-z
  • Li, Y., Thelen, K. M., Fernández, K. M., Nelli, R., Fardisi, M., Rajput, M., Trottier, N. L., Contreras, G. A. & Moeser, A. J. Developmental alterations of intestinal SGLT1 and GLUT2 induced by early weaning coincides with persistent low-grade metabolic inflammation in female pigs American Journal of Physiology-Gastrointestinal and Liver Physiology 322(3) G346-G359. https://doi.org/10.1152/ajpgi.00207.2021
  • Kuchipudi, S. V., Surendran-Nair, M., Ruden, R. M., Yon, M., Nissly, R. H., Vandegrift, K. J., Nelli, R. K., Li, L., Jayarao, B. M., Maranas, C. D., Levine, N., Willgert, K., Conlan, A. J. K., Olsen, R. J., Davis, J. J., Musser, J. M., Hudson, P. J. & Kapur, V. Multiple spillovers from humans and onward transmission of SARS-CoV-2 in white-tailed deer Proceedings of the National Academy of Sciences 119(6) https://doi.org/10.1073/pnas.2121644119
  • Nelli, R. K., Mora-Díaz, J. C. & Giménez-Lirola, L. G. The Betacoronavirus PHEV Replicates and Disrupts the Respiratory Epithelia and Upregulates Key Pattern Recognition Receptor Genes and Downstream Mediators, Including IL-8 and IFN-λ mSphere 6(6) https://doi.org/10.1128/msphere.00820-21
  • Nelli, R. K., Phadke, K., Castillo, G., Yen, L., Saunders, A., Rauh, R., Nelson, W., Bellaire, B. H. & Giménez-Lirola, L. G. Enhanced apoptosis as a possible mechanism to self-limit SARS-CoV-2 replication in porcine primary respiratory epithelial cells in contrast to human cells Cell Death Discovery 7(1) https://doi.org/10.1038/s41420-021-00781-w
  • Sang, Y., Miller, L. C., Nelli, R. K. & Giménez-Lirola, L. G. Harness Organoid Models for Virological Studies in Animals: A Cross-Species Perspective Frontiers in Microbiology 12 https://doi.org/10.3389/fmicb.2021.725074
  • Mora-Díaz, J. C., Piñeyro, P. E., Rauh, R., Nelson, W., Sankoh, Z., Gregg, E., Carrillo-Ávila, J. A., Shen, H., Nelli, R. K., Zimmerman, J. J. & Giménez-Lirola, L. G. Porcine Hemagglutinating Encephalomyelitis Virus Infection<i>In Vivo</i>and<i>Ex Vivo</i> Journal of Virology 95(12) https://doi.org/10.1128/jvi.02335-20
  • Kuchipudi, S. V., Nelli, R. K., Gontu, A., Satyakumar, R., Surendran Nair, M. & Subbiah, M. Sialic Acid Receptors: The Key to Solving the Enigma of Zoonotic Virus Spillover Viruses 13(2) 262. https://doi.org/10.3390/v13020262
  • Andres Contreras, G., De Koster, J., de Souza, J., Laguna, J., Mavangira, V., Nelli, R. K., Gandy, J., Lock, A. L. & Sordillo, L. M. Lipolysis modulates the biosynthesis of inflammatory lipid mediators derived from linoleic acid in adipose tissue of periparturient dairy cows Journal of Dairy Science 103(2) 1944-1955. https://doi.org/10.3168/jds.2019-17256
  • Albornoz, R., Sordillo, L., Contreras, G., Nelli, R., Mamedova, L., Bradford, B. & Allen, M. Diet starch concentration and starch fermentability affect markers of inflammatory response and oxidant status in dairy cows during the early postpartum period Journal of Dairy Science 103(1) 352-367. https://doi.org/10.3168/jds.2019-16398
  • Holz, C. L., Sledge, D. G., Kiupel, M., Nelli, R. K., Goehring, L. S. & Soboll Hussey, G. Histopathologic Findings Following Experimental Equine Herpesvirus 1 Infection of Horses Frontiers in Veterinary Science 6 https://doi.org/10.3389/fvets.2019.00059
  • Nelli, R. K., De Koster, J., Roberts, J. N., de Souza, J., Lock, A. L., Raphael, W., Agnew, D. & Contreras, G. A. Impact of uterine macrophage phenotype on placental retention in dairy cows Theriogenology 127 145-152. https://doi.org/10.1016/j.theriogenology.2019.01.011
  • De Koster, J., Nelli, R. K., Strieder-Barboza, C., de Souza, J., Lock, A. L. & Contreras, G. A. The contribution of hormone sensitive lipase to adipose tissue lipolysis and its regulation by insulin in periparturient dairy cows Scientific Reports 8(1) https://doi.org/10.1038/s41598-018-31582-4
  • Zarski, L. M., High, E. A., Nelli, R. K., Bolin, S. R., Williams, K. J. & Hussey, G. Development and application of a quantitative PCR assay to study equine herpesvirus 5 invasion and replication in equine tissues in vitro and in vivo Journal of Virological Methods 248 44-53. https://doi.org/10.1016/j.jviromet.2017.04.015
  • Holz, C. L., Nelli, R. K., Wilson, M. E., Zarski, L. M., Azab, W., Baumgardner, R., Osterrieder, N., Pease, A., Zhang, L., Hession, S., Goehring, L. S., Hussey, S. B. & Soboll Hussey, G. Viral genes and cellular markers associated with neurological complications during herpesvirus infections Journal of General Virology 98(6) 1439-1454. https://doi.org/10.1099/jgv.0.000773
  • Zarski, L., High, E., Nelli, R., Bolin, S., Williams, K. & Hussey, G. S. Development of a real-time PCR assay for quantification of equine herpesvirus 5 (EHV-5) and studying EHV-5 pathogenesis Journal of Equine Veterinary Science 39 S11-S12. https://doi.org/10.1016/j.jevs.2016.02.024
  • Holz, C., Wilson, M., Zarski, L., Nelli, R., Pease, A., Azab, W., Osterrieder, N., Goehring, L., Sledge, D., Kiupel, M. & Soboll Hussey, G. Evaluation of immunity and clinical disease following infection of horses with Equine herpesvirus-1 and mutants of differing neuropathogenic potential Journal of Equine Veterinary Science 39 S26-S27. https://doi.org/10.1016/j.jevs.2016.02.058
  • Nelli, R. K., Maes, R., Kiupel, M. & Hussey, G. S. Use of a feline respiratory epithelial cell culture system grown at the air–liquid interface to characterize the innate immune response following feline herpesvirus 1 infection Virus Research 214 39-48. https://doi.org/10.1016/j.virusres.2016.01.006
  • Kuchipudi, S. V., Tellabati, M., Nelli, R. K., White, G. A., Perez, B. B., Sebastian, S., Slomka, M. J., Brookes, S. M., Brown, I. H., Dunham, S. P. & Chang, K. 18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells Virology Journal 9(1) https://doi.org/10.1186/1743-422x-9-230
  • Baquero-Perez, B., Kuchipudi, S. V., Nelli, R. K. & Chang, K. A simplified but robust method for the isolation of avian and mammalian muscle satellite cells BMC Cell Biology 13(1) https://doi.org/10.1186/1471-2121-13-16
  • Nelli, R. K., Dunham, S. P., Kuchipudi, S. V., White, G. A., Baquero-Perez, B., Chang, P., Ghaemmaghami, A., Brookes, S. M., Brown, I. H. & Chang, K. Mammalian Innate Resistance to Highly Pathogenic Avian Influenza H5N1 Virus Infection Is Mediated through Reduced Proinflammation and Infectious Virus Release Journal of Virology 86(17) 9201-9210. https://doi.org/10.1128/jvi.00244-12
  • Kuchipudi, S. V., Dunham, S. P., Nelli, R., White, G. A., Coward, V. J., Slomka, M. J., Brown, I. H. & Chang, K. C. Rapid death of duck cells infected with influenza: a potential mechanism for host resistance to H5N1 Immunology &amp; Cell Biology 90(1) 116-123. https://doi.org/10.1038/icb.2011.17
  • Nelli, R. K., Kuchipudi, S. V., White, G. A., Perez, B. B., Dunham, S. P. & Chang, K. Comparative distribution of human and avian type sialic acid influenza receptors in the pig BMC Veterinary Research 6(1) https://doi.org/10.1186/1746-6148-6-4