Influenza virus, Group A (matrix) PCR (Avian, Swine)
Section: Molecular Diagnostics
Turnaround Time: 1 - 2 business days
Days Test Started: Monday,Tuesday,Wednesday,Thursday,Friday
Species: Avian, Canine, Chicken, Equine, Ovine, Porcine, Turkey
Swabs (species appropriate)
See Collection Information
8 - 10 grams
Oral Fluid (porcine only)
5 - 8 ml
For specifics on avian sample collection, please read the article from USDA Avian Sample Collection for Influenza A and Newcastle Disease. Please follow these guidelines and submit samples in the appropriate broth. Indicate the sample pooling method used on the submission form.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leak proof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
ORAL FLUID - Submit oral fluid in a leak proof container (i.e. plastic conical tube) with appropriate labeling.
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to firstname.lastname@example.org.
BULK - Please include bulk material inside the shipping box to prevent samples from moving or being crushed during transit (loose glass blood tube and an ice pack will always break).
For increased avian mortalities where Avian Influenza is concerned, please contact the ADDL and Indiana Board of Animal Health as soon as possible and before submitting.
Avian Influenza samples testing positive on the matrix PCR will be further tested for H5 and H7. Positive samples for either H5 or H7 will be sent to NVSL for confirmation.
Swine Influenza (IAV-S) samples testing positive within the parameters for the USDA Surveillance Program will be further tested (Subtyping, Virus Isolation, Sequencing H & N) using the sample with the lowest CT value at no additional charge to the client. To qualify, Lung and Nasal Swab samples must have a CT value less than 25 OR an Oral Fluid sample with a CT value less than 20. For more information, see Appendix C for the Influenza A in Swine Testing Algorithm. Lung samples can only be tested individually and not pooled.
- Indiana Animal Disease Diagnostic Laboratory Home
- Search Tests
- Lab Locations & Contacts
- About Us
- Submissions and Forms
- Online Test Results
- Policies & Fees
- Accession Submission Forms
- Indiana ADDL Submission Form Instructions.pdf
- Aquatic Submission Form.pdf
- Avian Health Submission Form.pdf
- Commercial Poultry Incident Form.pdf
- Companion Animal Submission Form.pdf
- Necropsy Submission Form.pdf
- Ruminant Submission Form.pdf
- Serology Submission Form.pdf
- Surgical Pathology/Biopsy Form.pdf
- Swine Herd Health Form.pdf
- Toxicology Submission Form.pdf
- Multiple Animal Continuation Form.xlsx
- New Client Information Form.pdf
- Submitting Veterinarian Release Form.pdf
- Supply Order Form.pdf