Bovine Respiratory Syncytial Virus (BRSV) PCR

Section: Molecular Diagnostics

Fee: $38.50

Turnaround Time: 2–8 business days

Days Test Started: Wednesday, Friday

Species: Bovine, Caprine, Ovine, Ruminant

Specimen

Name Container Minimum Amount
LungSealed, Leakproof Container or WhirlPack8 - 10 grams
Deep Nasal or Nasopharyngeal SwabViral Transport Medium/Leakproof Tube
Tracheobronchial lavageViral Transport Medium/Leakproof Tube

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name. SWABS: Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline/PBS. Any bacterial swab system (Amies agar, with or without charcoal) is unacceptable and will not be tested.

LUNG: Several samples of lesioned lung.

Refrigerate all samples until shipment.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information

Swabs from the nares or cranial nasal cavity have limited ability to identify BRSV infection.
Also available as part of the Bovine Respiratory Virus Screen PCR (BRSV, IBR, PI3, BCV, BVD)