Mycobacterium paratuberculosis (Johne’s) ELISA - Caprine and Ovine
Section: Serology
Fee: $6.60 ($4.00 > 25 samples)
Turnaround Time: 1–2 business days (Samples must arrive by Wednesday)
Days Test Started: Thursday
Species: Caprine, Ovine
Specimen
Name
Container
Minimum Amount
Serum
Red Top Vacutainer Tube or Equivalent
0.5-1 ml
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.’BULK - Please include bulk material inside the shipping box to prevent samples from moving or being crushed during transit (loose glass blood tube and an ice pack will always break).’SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
Additional Information
Tests will start at the ADDL West Lafayette location on Thursday. Samples must be received by the Wednesday prior. Contact the lab for rush and/or off schedule testing for an additional fee. This test is also available as part of the Small Ruminant Serology Panel. For serologic testing of cattle or sheep for Johne’s, please use, Johne’s ELISA - Bovine and Ovine.- Indiana Animal Disease Diagnostic Laboratory Home
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