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College of Veterinary Medicine

Mycobacterium paratuberculosis (Johne’s) ELISA - Caprine and Ovine

Section: Serology

Fee: $6.75 ($4.75 > 25 samples)

Turnaround Time: 1–2 business days

Days Test Started: Tuesday, Thursday

Species: Caprine, Ovine

Specimen

Name Container Minimum Amount
SerumRed Top Vacutainer Tube or Equivalent0.5-1 ml

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.’BULK - Please include bulk material inside the shipping box to prevent samples from moving or being crushed during transit (loose glass blood tube and an ice pack will always break).’SERUM - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.

Additional Information

Tests will start at the ADDL West Lafayette location on Tuesday and Thursday. Samples must be received by Monday and/or Wednesday prior. Contact the lab for rush and/or off-schedule testing for an additional fee. This test is also available as part of the Small Ruminant Serology Panel. For serologic testing of cattle for Johne’s, please use Johne’s ELISA - Bovine.

Quick Links

Accession Submission Forms