Equine Arteritis Virus (EAV) Virus Isolation
Section: Virology
Fee: $77.00
Turnaround Time: 2–3 weeks
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Equine
Specimen
Name
Container
Minimum Amount
Fetal tissue
Sealed, Leakproof Container or Whirlpak
1-10 g
Spleen, Lung
Sealed, Leakproof Container or Whirlpak
1-10g
Nasal Swab
Viral Transport Medium/Leakproof Tube
Whole Blood
Purple Top Vacutainer (EDTA)
5-10 ml
Semen
Sealed, Leakproof Container or Whirlpak
5-10 ml
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, and Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in appropriate vacutainer tube, making sure to meet minimum quantity.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label them with tissue type, animal ID, and owner name.
SEMEN - Submit semen in a leak-proof container (i.e., plastic conical tube) with appropriate labeling.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit the placenta (cotyledonary, NOT amniotic).
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture). DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap them securely with a paper towel or bubble wrap to provide protection.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
SEMEN - Ensure the container is securely sealed and labeled.
FETUS/FETAL TISSUES - Double bag body if shipping through a courier service. Ship the placenta in a separate leakproof plastic bag.
Additional Information
Virus isolation of Equine arteritis virus.- Indiana Animal Disease Diagnostic Laboratory Home
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