Bacterial 16s Sequencing
Section: Molecular Diagnostics
Fee: $85.00
Turnaround Time: 10–21 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: All Species
Specimen
| Name | Container | Minimum Amount |
|---|---|---|
| Bacterial Isolate | Culture plate or slant | |
| Formalin Fixed Paraffin Embedded Tissues | Sterile, Sealed, Leakproof Container or Whirlpak | Five - 10μm scrolls |
Collection Information
Please ensure the sample is a pure culture. Mixed cultures require additional isolation and identification at additional cost to the submitter. Scrolls of formalin fixed paraffin embedded tissues are most useful when a bacterial organism has been visualized by a pathologist.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu. Ship specimens overnight with an ice pack. DO NOT SHIP OVER THE WEEKEND OR HOLIDAY.
Additional Information
This method compares the highly conserved 16s ribosomal RNA sequence of the unknown isolate to that of known bacterial pathogens. When traditional methods of bacterial identification (biochemical tests, MALDI-TOF) fail, or when a culture is unavailable, 16s Sequencing may be a useful alternative.Quick Links
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Accession Submission Forms
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