Infectious Bovine Rhinotracheitis (IBR) Virus Isolation
Section: Virology
Fee: $40.00
Turnaround Time: 2–3 weeks
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Bovine, Caprine, Ovine, Ruminant
Specimen
Name
Container
Minimum Amount
Lung, Trachea, Bronchial Lymph Nodes
Sealed, Leakproof Container or Whirlpak
1-10g
Nasal Swab
Viral Transport Medium/Leakproof Tube
Kidney, Brain
Sealed, Leakproof Container or Whirlpak
1-10g
Fetal Tissues
Sealed, Leakproof Container or Whirlpak
1-10g
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, and Date.
REFRIGERATION - Refrigerate all samples until shipment.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label them with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit the placenta (cotyledonary, NOT amniotic).
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture). DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
Virus Isolation of Infectious Bovine Rhinotracheitis.- Indiana Animal Disease Diagnostic Laboratory Home
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