Toxoplasma gondii PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1 – 2 business days
Days Test Started: Monday
Species: Bovine, Canine, Caprine, Feline, Ovine, Porcine, Ruminant
Specimen
Name
Container
Minimum Amount
Whole Blood
EDTA Purple Top Vacutainer
2 - 5 ml
Feces (feline only)
Leakproof Container
8 - 10 grams
Intestine (feline only)
Leakproof Container or WhirlPak
2-3 segments
Brain
Leakproof Container or WhirlPak
8 - 10 grams
Spleen
Leakproof Container or WhirlPak
8 - 10 grams
Liver
Leakproof Container or WhirlPak
8 - 10 grams
Fetal lung, liver, splen
Leakproof Container or WhirlPak
8 -10 grams
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in appropriate vacutainer tube making sure to meet minimum quantity.
FECES - Collect feces in a leak proof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit placenta (cotyledonary NOT amniotic). Placenta (Cotyledon Only) - several samples in a leakproof container or whirlpak.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap securely with paper towel or bubble wrap to provide protection.
FECES - Ensure container is securely sealed. Double bag all diarrhea samples to contain any leaks.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
FETUS/FETAL TISSUES - Double bag body if shipping through a courrier service. Ship placenta in a separate leakproof, plastic bag.
Additional Information
This test is also part of the ruminant (bovine, ovine, caprine) abortion panels - see Ruminant Aborition LTN Multiplex PCR (Leptospira, Toxoplasma, and Neospora).- Indiana Animal Disease Diagnostic Laboratory Home
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