PVM Directory / Nelly O Elshafie
Molecular Research Associate
Animal Disease Diagnostic Laboratory
Purdue University College of Veterinary Medicine
625 Harrison Street
West Lafayette, IN 47907
765-494-7440
765-494-9181 (fax)
nelshafi@purdue.edu
As a postdoctoral researcher, I specialized in utilizing dogs as large animal models for immunoprevention of breast ductal carcinoma in situ. I have demonstrated advanced proficiency across a spectrum of essential skills. Since August 2023, I have exhibited adept handling and restraint techniques, extensive experience in conducting blood collection procedures, and expertise in administering injections for dogs. Additionally, I possess vast experience in evaluating humoral and cellular immune responses using techniques such as ELISA and ELISPOT, which provide valuable insights into immunological processes. Moreover, I am skilled in Western blotting techniques, enabling the detection and quantification of specific proteins, thus further enriching the molecular biology toolkit.
During my PhD journey, I have learned and mastered a robust skill set in molecular biology and diagnostics, such as DNA and RNA extraction, gel electrophoresis, PCR arrays, and nucleic acid sequencing, which enabled me to comprehend the characterization of biological samples in researching non-coding small RNA in canine Diffuse Large B-Cell Lymphoma (DLBCL), where I focused on identifying the biological significance of deregulated miRNAs driving the tumorigenic process. I propose ancillary diagnostic miRNA-based models for canine DLBCL, as published in Veterinary Pathology in 2021 and Frontiers in Oncology in 2023. Moreover, as published in FEBS Open Bio, 2024, I have investigated miRNA profiles associated with remission and survival rates. This work begins with developing diagnostic and prognostic tools to enhance therapeutic strategies.
Additionally, my experience extends to improving diagnostic approaches for various diseases, including fungal (as published in MDPI Veterinary Sciences 2022), bacterial (as published in Transboundary and Emerging Diseases 2021 and Journal of Wildlife Diseases 2024), and protozoal infections, using molecular tools extracted from biopsy tissues and body fluids. Furthermore, my expertise extends to bioinformatics analysis of RNA and sRNA sequencing data, enabling in-depth exploration of genetic information and molecular pathways.
2023 - PhD | Purdue University
2010 - One Year Diplomat Biochemistry | Benha University, Egypt
2005 - DVM | Benha University, Egypt
August 2023–August 2024 | Postdoctoral Research Associate, Dept. of Comparative Pathobiology, Purdue University
