Mohamed Seleem, DVM, MS, PhD

Department of Comparative Pathobiology

Associate Professor of Microbiology

Purdue University College of Veterinary Medicine
625 Harrison Street
West Lafayette, IN 47907

Phone: 765.494.0763


2006 - PhD | Virginia Tech
2000 - MS | Assiut University
1995 - DVM | Assiut University


2015 - | Associate Professor, College of Veterinary Medicine, Purdue University
2011 - 2015 | Assistant Professor, College of Veterinary Medicine, Purdue University
2006 - 2007 | Postdoctoral Research Associate: College of Veterinary Medicine, Cornell University
2003 - 2006 | Teaching and Research Assistant: VA-MD Regional College of Vet. Med. Virginia Tech
2001 - 2003 | Visiting Scholar, VA-MD Regional College of Vet. Med. Virginia Tech
1996 - 2001 | Assistant Lecturer and Research Scientist: Faculty of Veterinary Medicine, Assiut University, Egypt

Selected Publications

– S. Thangamani, M. Maland, H. Mohammad, P. Pascuzzi , L. Avramova, C. Koehler, T. Hazbun and M. N. Seleem. (2017). Repurposing Approach Identifies Auranofin with Broad Spectrum Antifungal Activity That Targets Mia40-Erv1 Pathway. Front. Cell. Infect. Microbiol. 18 January 2017

– Eman Yahia, Haroon Mohammad, Tamer M Abdelghany, Eman Fayed, Mohamed N Seleem, Abdelrahman S Mayhoub. (2017). Phenylthiazole antibiotics: A metabolism-guided approach to overcome short duration of action. European Journal of Medicinal Chemistry. 126:604-613

– Zahid Iqbal, Mohamed N Seleem, Hafiz Iftikhar Hussain, Lingli Huang, Haihong Hao, Zonghui Yuan. (2016). Comparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animals. Nature Scientific reports. 6: 35442

– Waleed Younis, Haroon Mohammad, Matthew Hostetler, Daneli López-Pérez, Calvin Steussy, Mark Lipton, Cynthia Stauffache, Serageldeen Sultan, Mohamed Wael Abd Al-Azeem, Asmaa AA Hussein, Mohamed N Seleem. (2016). Journal of Advanced Veterinary Research. (7)1;1-6

– Mostafa FN Abushahba, Asmaa AA Hussein, Mohamed N Seleem, Raafat Hassanein. (2016). Journal of Advanced Veterinary Research. (8)2;72-80

– S. Thangamani, H. Eldesouky, H. Mohammad, P. Pascuzzi, L. Avramova, T. Hazbun & M.N. Seleem. (2016). Ebselen exerts antifungal activity by regulating glutathione (GSH) and reactive oxygen species (ROS) production in fungal cells. Biochimica et Biophysica Acta (BBA) - General Subjects. 1861:1,A: 3002–3010

– A. Brezden, M. Mohamed, M. Nepal, J. Harwood, J. Kuriakose, M. N. Seleem, J. Chmielewski. (2016). Dual Targeting of Intracellular Pathogenic Bacteria with a Cleavable Conjugate of Kanamycin and an Antibacterial, Cell Penetrating Peptide. Journal of the American Chemical Society. DOI: 10.1021/jacs.6b04831, August 05, 2016

– A. AbdelKhalek, C. Ashby, B. Patel, T. Talele & M.N. Seleem. (2016). In Vitro Antibacterial Activity of Rhodanine Derivatives against Pathogenic Clinical Isolates. PLoS One. 2016 Oct 6;11(10):e0164227

– T. Maltais, A. Adak, W. Younis , M.N. Seleem, A. Wei. (2016). Label-Free Detection and Discrimination of Bacterial Pathogens Based on Hemin Recognition. Bioconjug Chem. 10.1021/acs.bioconjchem.6b00236.

– M. Abushahba, H. Mohammad, M.N. Seleem (2016). Targeting Multidrug-resistant Staphylococci with an anti-rpoA Peptide Nucleic Acid Conjugated to the HIV-1 TAT Cell Penetrating Peptide Nucleic Acid Conjugated to the HIV-1 TAT Cell Penetrating Peptide. Molecular Therapy—Nucleic Acids 5 (7), e339.

– M. Seleem, A. Disouky, H. Mohammad, T. Abdelghany, A. Mancy, S. Bayoumi, A. Elshafeey, A. El-Morsy, M. N. Seleem and A. S. Mayhoub. (2016). Second-Generation Phenylthiazole Antibiotics with Enhanced Pharmacokinetic Properties. J. Med. Chem. 26;59(10):4900-12.

– M. Mohamed, A. Abdelkhalek, M.N. Seleem. (2016). Evaluation of short synthetic antimicrobial peptides for treatment of drug-resistant and intracellular Staphylococcus aureus. Scientific Reports 6, Article number: 29707.

– W. Hong, C. Liao, H. Zhao, W. Younis, Y. Zhang, M. N. Seleem, and J. Cheng. (2016). In situ Detection of a Single Bacterium in Complex Environment by Hyperspectral CARS Imaging. ChemistrySelect:1;3, 513–517.

– S. Thangamani, H. Mohammad, M. Abushahba, T. Sobreira, V Hedrick, L. Paul & M. N. Seleem. (2016). Antibacterial activity and mechanism of action of auranofin against multi-drug resistant bacterial pathogens. Scientific Reports 6, Article number: 22571.

– S. Thangamani, H. Mohammad, M. Abushahba, T. Sobreira & M. N. Seleem. (2016). Repurposing auranofin for the treatment of cutaneous staphylococcal infections. Int J Antimicrob Agents.

– S. Thangamani, H. Mohammad, M. Abushahba, M. Hamed, T. Sobreira, V. Hedrick, L. Paul & M. N. Seleem. (2015). Exploring simvastatin, an antihyperlipidemic drug, as a potential topical antibacterial agent. Scientific Reports 5: 16407.

– M. Abushahba, H. Mohammad, S. Thangamani, A. Hussein & M. N. Seleem. (2016). Impact of different cell penetrating peptides on the efficacy of antisense therapeutics for targeting intracellular pathogens. Scientific Reports 6: 20832.

– H. Mohammad, M. Cushman , M. N. Seleem. 2015. Antibacterial Evaluation of Synthetic Thiazole Compounds In Vitro and In Vivo in a Methicillin-Resistant Staphylococcus aureus (MRSA) Skin Infection Mouse Model. PLoS One. 2015 Nov 4;10(11):e0142321.

– S. Thangamani, M. Nepal, J. Chmielewsk, M. N. Seleem. 2015. Antibacterial activity and therapeutic efficacy of Fl-PRPRPL-5, a cationic amphiphilic polyproline helix, in a mouse model of staphylococcal skin infection. Drug Design, Development and Therapy. 2015:9; 5749—5754.

– S. Thangamani, W. Younis, and M. N. Seleem. (2015). Repurposing celecoxib as a topical antimicrobial agent. Front Microbiol. 2015: 28; 6:750.

– S. Thangamani, W. Younis, and M. N. Seleem. (2015). Repurposing Clinical Molecule Ebselen to Combat Drug Resistant Pathogens. PLoS One.10 (7):e0133877.

– W. Younis, S. Thangamani, and M.N. Seleem. (2015). Repurposing Non-antimicrobial Drugs and Clinical Molecules to Treat Bacterial Infections. Current Pharmaceutical Design. 2015 May 6.

– Dabral N, Jain-Gupta N, M. N. Seleem, Sriranganathan N, Vemulapalli R. (2015). Overexpression of Brucella putative glycosyltransferase WbkA in B. abortus RB51 leads to production of exopolysaccharide. Front Cell Infect Microbiol. 24;5:54.

– Davis DC, Mohammad H, Kyei-Baffour K, Younis W, Creemer CN, M. N. Seleem, Dai M. (2015). Discovery and characterization of aryl isonitriles as a new class of compounds versus methicillin- and vancomycin-resistant. Eur J Med Chem. 16;101:384-390.

– S. Thangamani, W. Younis, and M. N. Seleem. (2015). Repurposing ebselen for treatment of multidrug-resistant staphylococcal infections. Scientific Reports. 26;5:11596

– M. Nepal, S. Thangamani, M. N. Seleem & J. Chmielewski. (2015). Targeting Intracellular Bacteria with an Extended Cationic Amphiphilic Polyproline Helix. Org. Biomol. Chem. 19;13 (21):5930-6

– H. Mohammad, PV Reddy, D. Montelenoe, AS Mayhoub, M. Cushman, GK Hammac, and M. N. Seleem. (2015). Antibacterial Characterization of Novel Synthetic Thiazole Compounds against Methicillin-Resistant Staphylococcus pseudintermedius. PLoS One. 10(6):e0130385

– Mohammad, H., Reddy, P.V.N., Monteleone, D., Cushman, M., & M. N. Seleem. (2015). Synthesis and Antibacterial Evaluation of A Novel Series of Synthetic Phenylthiazole Compounds Against Methicillin-resistant Staphylococcus aureus (MRSA). European Journal of Medicinal Chemistry. 94 (2015) 306-316.

– H. Mohammad, S. Thangamani & M. N. Seleem. (2015). Antimicrobial Peptides and Peptidomimetics - Potent Therapeutic Allies for Staphylococcal Infections. Current Pharmaceutical Design. 21 (16) 2073-2088.

– S. Thangamani, H. Mohammad, W. Younis & M. N. Seleem. (2015). Repurposing non-antimicrobial drugs for treatment of staphylococcal infections. Current Pharmaceutical Design. 21 (16) 2089-2100.

– M. F. Mohamed, G. K. Hammac, L. Guptill & M. N. Seleem (2014). Antibacterial Activity of Novel Cationic Peptides against Clinical Isolates of Multi-Drug Resistant Staphylococcus pseudintermedius from Infected Dogs. PloS one 9 (12), e116259.

– M. F. Mohamed & M. N. Seleem. (2014). Efficacy of short novel antimicrobial and anti-inflammatory peptides in a mouse model of methicillin-resistant Staphylococcus aureus (MRSA) skin infection. Drug Design, Development and Therapy (2014:8): 1979-1983.

– H. Mohammad, A. S. Mayhoub, A. Ghafor, M. Soofi, R. Alajlouni, M. Cushman & Seleem, M. N. (2014). Discovery and characterization of potent substituted thiazole compounds versus methicillin-and vancomycin-resistant Staphylococcus aureus. Journal of Medicinal Chemistry 57 (4): 1609-1615.

– M. F. Mohamed, M. I. Hamed, A. Panitch & M. N. Seleem. (2014).Targeting methicillin-resistant Staphylococcus aureus with short synthetic peptides: coupling membrane disruption with DNA binding. Antimicrobial Agent and Chemotherapy 58 (7): 4113-4122.

– H. Mohammad , A. S. Mayhoub, M. Cushman & M. N. Seleem. (2014). Anti-biofilm activity and synergism of novel thiazole compounds with glycopeptide antibiotics against multidrug-resistant Staphylococci. Journal of Antibiotic. doi:10.1038/ja.2014.142

– A. Athamneh, R. Alajlouni, R. S. Wallace, Seleem, M. N. & Ryan Senger. (2014). Phenotypic profiling of antibiotic response signatures in Escherichia coli using Raman spectroscopy. Journal of Antimicrobial Agent and Chemotherapy 58 (3): 1302-1314.

– W. Lv, B. Banerjee, K. Molland, Seleem , M. N., A. Ghafoor, B. Wan, S. Franzblau & A. Mesecar, M. Cushman. (2014). Synthesis and Antibacterial Activity of 3-(3-Aryl-pyrrolidin-1-yl)-5-aryl-1,2,4-triazine Inorganic Pyrophosphatase Inhibitors. Bioorganic & Medicinal Chemistry 22 (1): 406-18.

– E. A. Swanson, L. J. Freeman, Seleem, M. N. & P. W. Snyder. (2014). Biofilm-infected wounds in a dog: Case report and review of the literature. Journal of the American Veterinary Medical Association 244(6): 699-707

– J. Kuriakose, V. Hernandez-Gordillo, M. Nepal, A.a Brezden, V. Pozzi, Seleem, M. N., & J. Chmielewski. (2013). Targeting intracellular pathogenic bacteria with unnatural proline-rich peptides: coupling antibacterial activity with macrophage penetration. Angewandte Chemie International Edition, 2013. 52(37): p. 9664-7.

– Alajlouni, R.A. and M.N. Seleem. (2013). Targeting Listeria Monocytogenes rpoA and rpoD Genes Using Peptide Nucleic Acids. Nucleic Acid Ther, 2013. 23(5): p. 363-7.

– P. Rajesekaran, J. Alexander, Seleem, M. N., N. Jain, , N. Sriranganathan, A. Wattam, J. Setubal & S. M. Boyle. (2013). Peptide nucleic acids inhibit Brucella suis in pure culture and infected macrophages. International Journal of Antimicrobial Agents 41 (4): 358-62

– M. Soofi & Seleem, M. N. (2012). Targeting Salmonella essential genes with antisense peptide nucleic acid. Antimicrobial Agent and Chemotherapy 56 (12): 6407-9

– A. Ranjan, N. Pothayee, Seleem, M. N., S. M. Boyle, R. Kasimanickam, J. S. Riffle &N. Sriranganathan. (2012). Nanomedicine for intracellular therapy. FEMS Microbiology letters 332 (1):1-9.

– Rajasekaran P., Surendran N., Seleem, M. N., N. Sriranganathan, Schurig G. G. & Boyle S. M. (2011). Over-expression of homologous antigens in a leucine auxotroph of Brucella abortus strain RB51 protects mice against a virulent B. suis challenge. Vaccine 29 (17):3106-3110

– Jain N., Contreras A., Kimswade G., Seleem M.N., Boyle S.M., Sriranganathan N. (2011). Effect of entF deletion on iron acquisition and erythritol metabolism by Brucella abortus 2308.

– Surendran N, Zimmerman K, Seleem M. N., Sriranganathan N, Boyle SM, Hiltbold EM, Lawler H, Heid B, Witonsky SG. (2010). Ability of Brucella abortus rough vaccine strains to elicit DC and innate immunity in lung using a murine respiratory model.

– A. Ranjan, N. Pothayee, T. P. Vadala, Seleem M.N., E. Restis, N. Sriranganathan,J. S. Riffle and R. Kasimanickam. (2010). Efficacy of amphiphilic Development of pluronic P85 based core-shell nanostructures encapsulating gentamicin against an in-vitro Salm

– Ranjan, A., Seleem M.N., N Jain, N. Pothayee, N. Sriranganathan, J. S. Riffle, R. Kasimanickam. (2010). Drug delivery using novel nanoplexes against a Salmonella mouse infection model.

– H. Alqublan, Seleem M. N., S. M. Boyle, and N. Sriranganathan. (2010). Tightly regulated expression system for Ochrobactrum.

– Seleem M.N., S.M. Boyle and N. Sriranganathan. (2010). Brucellosis; A re-emerging zoonosis.

– Seleem, M.N., P. Munusamy, H. Alqublan, A. Ranjan, G. Pickrell, N. Sriranganathan. (2009). Silica-antibiotic hybrid for targeting Salmonella.

– D. Gillaspie, I. Perkins, K. Larsen, A. McCord, S. Pangonis, D. Sweger, Seleem M.N., N. Sriranganathan, and B. Anderson. (2009). Plasmid based system for high-level gene expression and antisense gene knockdown in Bartonella.

– Ranjan, A., N. Pothayee, Seleem M.N., N. Sriranganathan, R. Kasimanickam, M. Makris and J. S. Riffle. (2009). In-vitro trafficking and efficacy of core-shell nanostructures for treating intracellular Salmonella.

– P. Munusamy, Seleem M.N., N. Sriranganathan, G. Pickrell. (2009). Synthesis and characterization of Magnetic Silica Xerogels for Targeting Intracellular Pathogens.

– Ranjan, A., N. Pothayee, Seleem M.N., R. Tyler, N. Sriranganathan, R. Kasimanickam, M. Makris and J. S. Riffle. (2009). Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against in vivo intracellular Salmonella model.

– Seleem M.N., Jain N., Pothayee N., Ranjan A, Riffle J., Sriranganathan N. (2009). Streptomycin-Doxycycline nanoparticles for targeting Brucella melitensis infection.

– P. Munusamy, Seleem M.N., H. Alqublan, R. Tyler, N. Sriranganathan, G. Pickrell. (2009). Sol-gel derived Silica matrix as drug delivery system for intracellular pathogens.

– Seleem M.N., N. Jain, H. Alqublan, R. Vemulapalli, S. M. Boyle, and N. Sriranganathan. (2008). Activity of native vs. synthetic promoters in Brucella.

– Rajasekaran, P., Seleem M.N., A. Contreras, N. Sriranganathan and S. Boyle. (2008). A leucine auxotroph of Brucella abortus strain RB51 vaccine as an environmentally safe vector for plasmid maintenance and antigen expression.

– Seleem M.N., M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Reporter genes for real-time in vivo monitoring of Ochrobactrum anthropi infection.

– Pothayee, N., M. L. Vadala, A. Ranjan, N. Jain, Seleem M.N., N. Sriranganathan and J. S. Riffle. (2008). Aminoglycoside-Ionopolymeric nanoplexes for targeting intracellular bacterial pathogens.

– Seleem M.N., M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Vectors for enhanced gene expression and protein purification in Salmonella.

– Sandal, I., S., Seleem M.N., Elswaifi, N. Sriranganathan and T. Inzana. (2008). Efficient expression vectors for Histophilus somnus.

– Seleem M.N., P. Rajasekaran, M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Simple Method for Transformation of Ochrobactrum anthropi.

– Seleem M.N., S.M. Boyle and N. Sriranganathan. (2008). Brucella; a pathogen without classical virulence genes.

– Seleem M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. (2007). Enhanced expression, detection and purification of recombinant protein using tandem fusion tags RNA stem loop.

– Seleem M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. (2007). High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element.

– Seleem M.N., M. ALI, S.M. Boyle, and N. Sriranganathan. (2006). Establishment of gene expression system in Ochrobactrum species.

– Contreras, A., Seleem M.N, S. M. Boyle, G. Schurig, N. Sriranganathan and A. Lopez-merino. (2006). Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis.

– Bandara, A., Seleem M.N, C. Jordan, D. Lindsay, G. Schurig, and N. Sriranganathan. (2006). Brucella abortus Strain RB51 can be used to Express Potentially Protective Antigens of Toxoplasma gondii.

– Seleem M.N., R. Vemulapalli, S.M. Boyle, G.G. Schurig and N. Sriranganathan. (2004). Improved expression vector for Brucella species.

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