CPB 697 RESEARCH SEMINAR

 

 

 

DEPARTMENT OF COMPARATIVE PATHOBIOLOGY

 

 

Hyung Wook Lim, M.S.

Graduate Student in Biochemistry & Molecular Biology

Department of Comparative Pathobiology

Purdue University

 

 

Suppression Of Humoral Immune Responses By FOXP3+ T Cells

And Regulation Of Chemokine Receptor Expression In FOXP3+ T Cells

 

 

Thursday, March 8, 2007

VPTH 112

3:30 p.m.

 

Abstract:

FOXP3 is a member of fork-head/winged-helix family of transcription factors expressed in the nucleus of regulatory T cells (FOXP3+ T cells). FOXP3+ T cells play a central role in regulation of humoral immune responses and prevention of autoimmunity by suppressing proliferation, activation, and function of immune cells.

It has been known that chemokine receptors play an important role in guiding cell migration during immune responses/homeostasis in our body.

However, it has been unclear how FOXP3+ T cells migrate to follicles (B cell area of secondary lymphoid tissue), whether they suppress B cell immune responses, and how chemokine receptors expression is regulated in FOXP3+ T cells.

We found that upon activation, they acquire migration capacity toward B cell area of secondary lymphoid tissue by up-regulating expression of B cell zone chemokine receptor CXCR5. In the follicle, FOXP3+ T cells efficiently suppress not only T cells and T cell driven B cell immune responses, but also they can directly suppress B cell and B cell immune responses.

We also found that chemokine receptors are differentially expressed on the surface of FOXP3+ T cells depending on the stage of differentiation and activation. FOXP3+ T cells are divided into naïve and memory types based upon CD45RA and CD45RO expression. Naïve FOXP3+ T cells mainly express secondary lymphoid tissue homing receptors, while memory FOXP3+ T cells express non-lymphoid tissue and lymphoid tissue homing receptors. Upon activation and differentiation, naïve FOXP3+ T cells change their chemokine receptor expression pattern from naïve to memory type.

Our result showed that potential mechanism of FOXP3+ T cells in follicles of secondary lymphoid tissue and the regulation of trafficking receptors in FOXP3+ T cells in human body.