Purdue University

Dr. Mohamed Seleem Assistant Professor of Veterinary Microbiology Purdue Veterinary Medicine Purdue University West Lafayette, IN 47907 Phone: 765-494-0763 Email: mseleem@purdue.edu

Education

Professional Experience

Research Interests

Targeted drug delivery for intracellular pathogens
Intracellular pathogens like Mycobacterium, Brucella, Salmonella and Listeria have developed various mechanisms to evade host defenses, and they can establish chronic infections. Treatment and eradication are difficult since infections are localized within phagocytic cells and most antibiotics, although highly active in-vitro, do not actively pass through cellular membranes. My research is focused on site-specific targeted drug delivery with increased bioavailability of antimicrobials/drugs using nanoparticles as carriers.

Awards and Honors

Publications

Book Chapters

Genome Mapping in Animals and Microbes, Volume 8- Animal Associated Microbes (Springer -2009) ISBN: 978-3-540-74040-7.

Selected Publications

1. Jain N., Contreras A., Kimswade G., Seleem M.N., Boyle S.M., Sriranganathan N. (2011). Effect of entF deletion on iron acquisition and erythritol metabolism by Brucella abortus 2308. "FEMS Microbiological Letters" Mar;316(1):1-6.

2. Surendran N, Zimmerman K, Seleem M. N., Sriranganathan N, Boyle SM, Hiltbold EM, Lawler H, Heid B, Witonsky SG. (2010). Ability of Brucella abortus rough vaccine strains to elicit DC and innate immunity in lung using a murine respiratory model. "Vaccine". Oct;28(43):7009-15.

3. A. Ranjan, N. Pothayee, T. P. Vadala, Seleem M.N., E. Restis, N. Sriranganathan,J. S. Riffle and R. Kasimanickam. (2010). Efficacy of amphiphilic Development of pluronic P85 based core-shell nanostructures encapsulating gentamicin against an in-vitro Salmonella and Listeria intracellular infection model. Journal of Antimicrobial Agents and Chemotherapy". Aug;54(8):3524-6.

4. Ranjan, A., Seleem M.N., N Jain, N. Pothayee, N. Sriranganathan, J. S. Riffle, R. Kasimanickam. (2010). Drug delivery using novel nanoplexes against a Salmonella mouse infection model. "Journal of Nanoparticle Research". May (12):905–914.

5. H. Alqublan, Seleem M. N., S. M. Boyle, and N. Sriranganathan. (2010). Tightly regulated expression system for Ochrobactrum. "Current Microbiology", Apr; 60(4):242-7.

6. Seleem M.N., S.M. Boyle and N. Sriranganathan. (2010). Brucellosis; A re-emerging zoonosis. "Veterinary Microbiology". Jan;140(3-4):392-8.2010

7. Seleem, M.N., P. Munusamy, H. Alqublan, A. Ranjan, G. Pickrell, N. Sriranganathan. (2009). Silica–antibiotic hybrid for targeting Salmonella. "Antimicrobial Agents and Chemotherapy" Oct; 53(10):4270-4.

8. D. Gillaspie, I. Perkins, K. Larsen, A. McCord, S. Pangonis, D. Sweger, Seleem M.N., N. Sriranganathan, and B. Anderson. (2009). Plasmid based system for high-level gene expression and antisense gene knockdown in Bartonella. "Applied and Environmental Microbiology". Aug;75(16):5434-6.

9. Ranjan, A., N. Pothayee, Seleem M.N., N. Sriranganathan, R. Kasimanickam, M. Makris and J. S. Riffle. (2009). In-vitro trafficking and efficacy of core-shell nanostructures for treating intracellular Salmonella. "Antimicrobial Agents and Chemotherapy". Sep;53(9):3985-8.

10. P. Munusamy, Seleem M.N., N. Sriranganathan, G. Pickrell. (2009). Synthesis and characterization of Magnetic Silica Xerogels for Targeting Intracellular Pathogens. "Journal of Bionanoscience". Jun;3 (1):16–21.

11. Ranjan, A., N. Pothayee, Seleem M.N., R. Tyler, N. Sriranganathan, R. Kasimanickam, M. Makris and J. S. Riffle. (2009). Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against in vivo intracellular Salmonella model. "International Journal of Nanomedicine" Dec;(4): 289-297.

12. Seleem M.N., Jain N., Pothayee N., Ranjan A, Riffle J., Sriranganathan N. (2009). Streptomycin-Doxycycline nanoparticles for targeting Brucella melitensis infection. "FEMS Microbiology Letters". 294(1): 24-31.

13. P. Munusamy, Seleem M.N., H. Alqublan, R. Tyler, N. Sriranganathan, G. Pickrell. (2009). Sol-gel derived Silica matrix as drug delivery system for intracellular pathogens. "Material Science and Engineering C". Oct;29( 8): 2313-18.

14. Seleem M.N., N. Jain, H. Alqublan, R. Vemulapalli, S. M. Boyle, and N. Sriranganathan. (2008). Activity of native vs. synthetic promoters in Brucella. "FEMS Microbiological Letters". 288 (2): 211-5.

15. Rajasekaran, P., Seleem M.N., A. Contreras, N. Sriranganathan and S. Boyle. (2008). A leucine auxotroph of Brucella abortus strain RB51 vaccine as an environmentally safe vector for plasmid maintenance and antigen expression. "Applied and Environmental Microbiology". 74 (22): 7051-5.

16. Seleem M.N., M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Reporter genes for real-time in vivo monitoring of Ochrobactrum anthropi infection. "FEMS Microbiological letters". 286:124-9.

17. Pothayee, N., M. L. Vadala, A. Ranjan, N. Jain, Seleem M.N., N. Sriranganathan and J. S. Riffle. (2008). Aminoglycoside-Ionopolymeric nanoplexes for targeting intracellular bacterial pathogens. "Polymer Preprints". 49 (2): 1036.

18. Seleem M.N., M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Vectors for enhanced gene expression and protein purification in Salmonella. "GENE". 421: 95-8.

19. Sandal, I., S., Seleem M.N., Elswaifi, N. Sriranganathan and T. Inzana. (2008). Efficient expression vectors for Histophilus somnus. "Microbiological Methods". 74:106-9. 2008.

20. Seleem M.N., P. Rajasekaran, M. Ali, S.M. Boyle and N. Sriranganathan. (2008). Simple Method for Transformation of Ochrobactrum anthropi. "World Journal of Microbiology and Biotechnology" 24: 2111-4.

21. Seleem M.N., S.M. Boyle and N. Sriranganathan. (2008). Brucella; a pathogen without classical virulence genes. "Veterinary Microbiology". 25:1-14.

22. Seleem M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. (2007). Enhanced expression, detection and purification of recombinant protein using tandem fusion tags RNA stem loop. "Applied Microbiology and Biotechnology. 75:1385-92.

23. Seleem M.N., M. Ali, M. Abd Alazim, S. Boyle and N. Sriranganathan. (2007). High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element. "Applied Microbiology and Biotechnology". 73:1123-7.

24. Seleem M.N., M. ALI, S.M. Boyle, and N. Sriranganathan. (2006). Establishment of gene expression system in Ochrobactrum species. "Applied and Environmental Microbiology". 72: 6833-6.

25. Contreras, A., Seleem M.N, S. M. Boyle, G. Schurig, N. Sriranganathan and A. Lopez-merino. (2006). Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis. "FEMS Immunology and Medical Microbiology". 48: 252-6.

26. Bandara, A., Seleem M.N, C. Jordan, D. Lindsay, G. Schurig, and N. Sriranganathan. (2006). Brucella abortus Strain RB51 can be used to Express Potentially Protective Antigens of Toxoplasma gondii. "J. Eukaryot. Microbiol". 53(S1): S166–8.

27. Seleem M.N., R. Vemulapalli, S.M. Boyle, G.G. Schurig and N. Sriranganathan. (2004). Improved expression vector for Brucella species. "BioTechniques". 37: 740-44.