- September 27 - October 1, 2016 - 2016 Purdue Veterinary Conference
- October 7, 2016 - VCS Seminar: Dr. Carrie Fulkerson, Dr. Bergstrom, and Dr. Ambrosius
- November 3-4, 2016 - Centers for the Human-Animal Bond Conference
- November 13-14, 2016 - Pet Bird Symposium
Purdue Veterinary Conference is designed to provide continuing education (CE) opportunities for the entire veterinary team. Attendees can earn up to 32 hours of CE. The 2016 Conference will offer new speakers, workshops and special events including a Veterinary Technology 40th Anniversary celebration.
These Seminars are given together and are worth One (1) Continuing Education (CE) credit.
"Optimize Computed Tomography Protocol for Alpaca Dentition"
Presenter: Dr. Carrie Fulkerson, 2nd Year Resident, Diagnostic Imaging
Introduction/Purpose: Radiographs are commonly used in alpacas with dental disease to identify which teeth are involved and determine the extent of osteomyelitis, sequestration and draining tracts. In spite of the frequency of dental disease in alpacas, there is a lack of information regarding optimal technical imaging parameters to perform dental CT. Optimal dental CT studies in alpacas are important to allow clear visualization of the tooth roots and surrounding alveolar bone. We hypothesized that optimal dental images would be obtained using helical acquisitions and 1.25 mm slice thickness in alpacas in sternal recumbency under injectable anesthesia scanned in a 64-slice CT scanner.
Methods: Three healthy adult alpacas were recruited for this prospective pilot study. Each alpaca was scanned in sternal recumbency under injectable anesthesia (ketamine, xylazine, and butophanol combination) using a 64-slice CT scanner with the following six protocols: (H1.25) helical, 1.25 mm slice thickness; (H2.5) helical, 2.5 mm slice thickness; (H5) helical, 5 mm slice thickness; (S1.25) sequential, 1.25 mm slice thickness; (S2.5) sequential, 2.5 mm slice thickness; (S5) sequential, 5 mm slice thickness. Five images of each alpaca were evaluated for each protocol and scored by three board-certified radiologists based on visualization of tooth roots, sharpness of tooth roots and image noise. A numeric scoring system was devised that yielded a higher score for the better images. Images were selected by the first author who was responsible for recording the scores but not involved in the evaluation. All images were reviewed using commercially available viewing software with a fixed window level and width and high frequency reconstruction algorithm. Statistical analysis of the scores was performed using ordered logistic regression models.
Results: The 1.25 mm slice thickness protocols scored higher than the 2.5mm and 5 mm slice thickness protocols for both tooth root visibility and tooth root sharpness with statistically significant differences (P< 0.001 for all). Sequential images scored higher than helical images for image noise with differences detected between the H1.25 protocol and S1.25, S2.5 and S5 protocols (P=0.003, P=0.003 and P=0.057, respectively). There was no significant difference between the H1.25 and S1.25 protocols with tooth root visibility or sharpness; however when image noise is evaluated, S1.25 scored significantly higher than H1.25.
Discussion/Conclusion: The recommended optimal protocol for alpaca dentition is S1.25 using a high frequency reconstruction algorithm. Alpaca skull evaluation performed under injectable anesthesia while positioned in sternal recumbency with a 64 slice CT scanner produced high quality images.
"Antibacterial Activity and Safety of Commercial Cationic Steroid Antibiotics and Neutral Superoxidized Water"
Presenter: Dr. Ben Bergstrom, 3rd Year Resident, Ophthalmology
Purpose. To investigate the antibacterial activity and safety of three topical ophthalmic products (CeragynTM and PurishieldTM – CSA Biotechnologies, Spanish Fork, UT; Vetericyn Plus – Vetericyn, Rialto, CA). Methods. Minimum inhibitory concentrations (MIC) in µg/ml were determined against 13 antibiotic resistant, ocular, bacterial isolates. For exposure assays, log 106 or log 109 colony forming units/ml (CFU) of Gram-positive and Gram-negative isolates were exposed to the products for 5 minutes simulating an eye drop’s contact time. In cytotoxicity assays, the products at varying concentrations were evaluated with a murine macrophage cell line. Commercial ophthalmic solutions and analytic grade antibiotics were positive controls. The mean and standard deviation were calculated for the exposure assay data. Cytotoxicity data were analyzed using one-way ANOVA, with post hoc Holm-Sidak’s multiple comparisons test. P-values < 0.05 were significant. Results. For MIC, Vetericyn Plus showed no inhibition for any organisms, while CeragynTM and PurishieldTM showed inhibition similar to controls. For the exposure assays, all 3 products inhibited all growth at the lower density. Vetericyn Plus did not inhibit growth at the higher CFU. CeragynTM and PurishieldTM demonstrated moderate bactericidal activity while the positive control completely inhibited growth of the high inoculum Gram-negative isolate. No bactericidal activity was demonstrated by any product or the positive control against the high inoculum Gram-positive isolate. Vetericyn Plus exhibited nonsignificant toxicity while CeragynTM and PurishieldTM exhibited significant toxicity. Conclusions. Vetericyn Plus had minimal antimicrobial activity in these assays, while CeragynTM and PurishieldTM had antibacterial and cytotoxicity profiles comparable to common topical ophthalmic antibiotics.
"Expression of Programmed Cell Death Ligand 1 in Canine Diffuse B-cell Lymphoma"
Presenter: Dr. Lisbeth Ambrosius, 3rd Year Resident, Oncology
The expression of programmed cell death ligand 1 (PD-L1) has been noted in a subset of human cancers. In many of these cancers, including diffuse large B-cell lymphoma, PD-L1 expression (PD-L1+) is correlated with a poor prognosis. Currently, there are numerous monoclonal antibodies directed against PD-L1 that are achieving clinical benefit in various human cancers. The objectives of this study in dogs were three-fold: 1) to quantify expression of PD-L1 in canine diffuse large B-cell lymphoma utilizing real-time quantitative polymerase chain reaction; 2) to determine whether PD-L1 expression is correlated to prognosis in terms of progression free survival; and 3) to determine if PD-L1 expression levels change after treatment with the CHOP protocol (cyclophosphamide, doxorubicin, vincristine, prednisone).
The Centers for Human-Animal Bond (CHAB) Conference is a prestigious event to unite leaders from University centers and institutes focused on research, teaching, and practice related to human-animal interaction. It was first convened 10 years ago and led to a special issue publication in the Journal of Veterinary Medical Education. Over the past decade there have been tremendous advances in the field. To connect and advance key leaders in this multi-disciplinary field, Purdue University’s College of Veterinary Medicine will host the second occurrence of this special, invitation-only event. The first day will be open to the public and feature a lecture and workshops on cutting edge methodology in the field. The second day will consist of interactive discussion and presentations regarding the role, progress, and future trajectory of centers and institutes in advancing the human-animal bond field through collaborative research and education. Attendees for day two will be invited leaders from centers and institutes focused on human-animal bond research and teaching at Universities throughout the United States.
This conference has a limited number of seats, don't wait- Register Now!
The two-day biennial Pet Bird Symposium returns November 13 - 14 featuring four guest speakers addressing a variety of topics for veterinarians, veterinary technicians and aviculturists. Topics include, “Commonly Kept Parrot Species and Their Characteristics,” “Update on Viral Diseases and Chlamydophila,” “Sedation, High Risk Anesthesia and Critical Care Management,” “Cloacal Prolapse Management,” “Dermatologic Conditions,” “The Geriatric Parrot,” and “Renal System Disorders.”
Speakers include Purdue Veterinary Medicine clinicians: Dr. Steve Thompson, clinical associate professor and director of the Pet Wellness Clinic, and Dr. Lorraine Corriveau, wellness clinician in the Small Animal Community Practice; as well as Dr. Angela Lennox, PVM adjunct assistant professor and avian specialist in Carmel, Ind., and Dr. Bianca Zaffarano, Iowa State University College of Veterinary Medicine faculty clinician.
In addition to lectures, the program will include a variety of case-based discussions. Single day registration options will also be available. Additional program and registration information coming soon.
Nine continuing education (CE) credits will be offered for this symposium.
Check back soon for registration and the preliminary program for this event!